Invitrogen

Galectin 1 Polyclonal Antibody

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Galectin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Galectin 1 using anti-Galectin 1 antibody (Product # PA5-95333) . Galectin 1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Galectin 1 antibody (Product # PA5-95333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Galectin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Galectin 1 in paraffin-embedded mouse intestine cancer tissues. Samples were incubated with Galectin 1 polyclonal antibody (Product # PA5-95333) at a 1 µg/mL dilution, and developed with Strepavidin-Biotin-Complex.

Galectin 1 Antibody in Western Blot (WB) — Western blot analysis of Galectin 1 in rat testis extract (lane 1), mouse spleen extract (lane 2), HeLa whole cell lysates (lane 3) and SMMC whole cell lysates (lane 4). Samples were incubated with Galectin 1 polyclonal antibody (Product # PA5-95333) using a 0.5 µg/mL dilution. developed was performed using enhanced chemiluminescence (ECL).

Galectin 1 Antibody in Flow Cytometry (Flow) — Flow Cytometry of Galectin 1 in PC-3 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Galectin 1 Polyclonal Antibody (Product # PA5-95333) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

产品信息

PA5-95333

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Galectin 1 recombinant protein (Position: A2-D135).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807136

产品详细信息

Human Galectin 1 shares 88% and 90% amino acid (aa) sequence identity with mouse and rat Galectin 1, respectively.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 14 kDa laminin-binding protein; 14 kDa lectin; beta-galactoside binding protein; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; Gal-1; Galaptin; Galectin-1; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactose binding, soluble 1; lectin, galactoside binding soluble 1; lectin, galactoside-binding, soluble, 1; pm12; Putative MAPK-activating protein PM12; RL 14.5; S-Lac lectin 1

基因别名: AA410090; Gal-1; GAL1; Galbp; galectin-1; GBP; L-14.5; L14; Lect14; LGALS1

UniProt ID: (Human) P09382, (Mouse) P16045, (Rat) P11762

Entrez Gene ID: (Human) 3956, (Mouse) 16852, (Rat) 56646

分子生物学功能: extracellular matrix protein