Invitrogen

Galectin 1 Monoclonal Antibody (6C8.4-1)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. 查看细节

2个已发表图片

5篇引用文献

查看其他19个Galectin 1抗体

说明书

Protocols

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说明书

Protocols

技术支持

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Galectin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Galectin 1 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membrane localization of Galectin 1 in MDA-MB-231. Panel e represents MCF7 cells showing negative staining for the same. Panel f represents control MDA-MB-231 cells with no primary antibody to assess background. The images were captured at 60X magnification.

Galectin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Galectin 1 showing staining in the cytoplasm and nucleus of paraffin-embedded human esophagus tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Galectin 1 Monoclonal Antibody (Product # 43-7400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Galectin 1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400) and a 15kDa band corresponding to Galectin 1 was observed across PC-3, THP-1, MDA-MB-231 but not in LNCaP and MCF7 and in Mouse Adipose. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), LNCaP (Lane 2), THP-1 (Lane 3), MDA-MB-231 (Lane 4), MCF7 (Lane 5) and tissue extracts (30 ug lysate) of Mouse Adipose (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Increased expression of Galectin-1 is seen in triple negative breast cancer (TNCB) (like MDA-MB-231) for promoting metastasis but not in non-TNBC like MCF7 [10.18632/oncotarget.16208] and in androgen unresponsive PC-3 cells in comparison to androgen responsive LNCaP [10.1155/2013/519436]. The band at ~25 kDa in Mouse Adipose corresponds to circulating IgGs that are commonly detected in mouse tissue lysates.

Galectin 1 Antibody — Figure 6 Hypoxia causes exacerbation of fibrosis via galectin-1 in mice injured with low levels of bleomycin. ( A ) Experimental design depicting two bleomycin insults (day 0--D0; day 7--D7) followed by hypoxia treatment for 3 weeks (day 8 to day 28). Galectin-1 inhibitor (OTX008) treatment was performed from day 5 to day 28. ( B ) Hypoxia treatment of mice injured with bleomycin induced fibrosis. Mice were exposed to hypoxia alone (m-p) or treated with intratracheal bleomycin alone (e-h) or underwent hypoxia and bleomycin insults together (q-t). Mice were also treated with galectin-1 inhibitor (i-l, u-x) or saline (e-h, q-t). H&E staining (a, e, i, m, q, u), Masson's trichrome staining for visualization of collagen deposition (b, f, j, n, r, v), alpha -SMA staining (c, g, k, o, s, w), and galectin-1 staining (d, h, l, p, t, x) was performed. Black scale bars=100 mu m; blue scale bars=20 mu m. alpha -SMA expression is increased in the proximal parenchyma (s; arrows) and distal parenchyma (s, inset) of mouse lungs treated with hypoxia and bleomycin. Similarly, galectin-1 expression is increased in proximal parenchyma (t; arrow and right bottom inset) and distal parenchyma (left bottom inset). Galectin-1 inhibition reversed lung remodeling (u), collagen deposition (v), and reduced alpha -SMA (w) and galectin-1 expression (x). ( C ) Galectin-1 inhibition reduced total collagen deposition in the lungs of mice injured with hypoxia and bleomycin. Total collagen was measured using s {TM}

Galectin 1 Antibody in Immunohistochemistry (IHC) — Figure 6 Hypoxia causes exacerbation of fibrosis via galectin-1 in mice injured with low levels of bleomycin. ( A ) Experimental design depicting two bleomycin insults (day 0--D0; day 7--D7) followed by hypoxia treatment for 3 weeks (day 8 to day 28). Galectin-1 inhibitor (OTX008) treatment was performed from day 5 to day 28. ( B ) Hypoxia treatment of mice injured with bleomycin induced fibrosis. Mice were exposed to hypoxia alone (m-p) or treated with intratracheal bleomycin alone (e-h) or underwent hypoxia and bleomycin insults together (q-t). Mice were also treated with galectin-1 inhibitor (i-l, u-x) or saline (e-h, q-t). H&E staining (a, e, i, m, q, u), Masson's trichrome staining for visualization of collagen deposition (b, f, j, n, r, v), alpha -SMA staining (c, g, k, o, s, w), and galectin-1 staining (d, h, l, p, t, x) was performed. Black scale bars=100 mu m; blue scale bars=20 mu m. alpha -SMA expression is increased in the proximal parenchyma (s; arrows) and distal parenchyma (s, inset) of mouse lungs treated with hypoxia and bleomycin. Similarly, galectin-1 expression is increased in proximal parenchyma (t; arrow and right bottom inset) and distal parenchyma (left bottom inset). Galectin-1 inhibition reversed lung remodeling (u), collagen deposition (v), and reduced alpha -SMA (w) and galectin-1 expression (x). ( C ) Galectin-1 inhibition reduced total collagen deposition in the lungs of mice injured with hypoxia and bleomycin. Total collagen was measured us

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:1,000

View 2 publications 2 publications

免疫组化 (IHC)

View 1 publication 1 publication

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

View 1 publication 1 publication

其他PubMed (Misc)

View 1 publication 1 publication

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6C8.4-1

抗原

Mixture of two peptides from the middle and C-terminal regions of human Galectin-1 protein

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2533538

产品详细信息

The peptides used for immunization have ~90% similarity to swine, rat, ovine, Chinese hamster sequences and 80% similar to bovine and mouse sequences.

靶标信息

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 14 kDa laminin-binding protein; 14 kDa lectin; beta-galactoside binding protein; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; Gal-1; Galaptin; Galectin-1; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside binding soluble 1; lectin, galactoside-binding, soluble, 1; pm12; Putative MAPK-activating protein PM12; S-Lac lectin 1

基因别名: AA410090; Gal-1; GAL1; Galbp; galectin-1; GBP; L-14.5; L14; Lect14; LGALS1

UniProt ID: (Human) P09382, (Mouse) P16045

Entrez Gene ID: (Human) 3956, (Mouse) 16852

分子生物学功能: extracellular matrix protein