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Gemin 2 Antibody (MA1-5777) in ICC/IF
Immunofluorescence analysis of Gemin 2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gemin 2 Mouse Monoclonal Antibody (Product # MA1-5777) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More
产品信息
MA1-5777
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Xenopus
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2E17
抗原
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2187799
产品详细信息
MA1-5777 detects Gemin2 in human and xenopus samples.
MA1-5777 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. MA1-5777 detects a band of approximately 34 kDa.
The MA1-5777 immunogen is His-Gemin2 (starting at 2nd methionine).
Store at 4ºC for one month, or in small aliquots at -20ºC for longer periods.
靶标信息
SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Component of gems 2; gem (nuclear organelle) associated protein 2; Gem-associated protein 2; Gemin-2; SIP 1; SIP-1; SIP1 delta; SMN interacting protein 1-delta; SMN-interacting protein 1; survival of motor neuron protein interacting protein 1; Survival of motor neuron protein-interacting protein 1; survivor of motor neuron protein interacting protein 1
基因别名: 1700012N19Rik; GEMIN2; SIP1; SIP1-delta; XELAEV_18042638mg
UniProt ID: (Human) O14893, (Mouse) Q9CQQ4
Entrez Gene ID: (Human) 8487, (Mouse) 66603, (Xenopus) 494588
