Invitrogen

GluR2 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他18个GluR2抗体

说明书

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说明书

Protocols

技术支持

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GluR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GluR2 on paraffin-embedded mouse brain tissue. Sample was incubated with GluR2 polyclonal antibody (Product# PA5-79326).

GluR2 Antibody in Western Blot (WB) — Western blot was performed using GluR2 Polyclonal Antibody (Product # PA5-79326) and a 100kDa band corresponding to GluR2 was observed across Mouse and Rat tissues tested except Mouse Liver. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Rat Brain (Lane 3) and Mouse Liver (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GluR2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of GluR2 was observed in Mouse Brain, Mouse Cerebellum and Rat Brain in comparison to Mouse Liver using product (Product # PA5-79326) in western blot. {RE}

产品信息

PA5-79326

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	-
免疫组化（石蜡） (IHC (P))	0.5-1 µg/mL	-
免疫组化（冰冻） (IHC (F))	0.5-1 µg/mL	-
免疫细胞化学 (ICC/IF)	0.5-1 µg/mL	-
流式细胞分析 (Flow)	1-3 µg/1x10^6 cells	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	E.coli-derived human GRIA2 recombinant protein (Position: N25-I360).
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 5mg BSA
内含物	0.05mg sodium azide
保存条件	-20°C
RRID	AB_2746442

产品详细信息

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

靶标信息

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: AMPA selective glutamate receptor; AMPA-selective glutamate receptor 2; GluA2; GluR-2; GluR-B; GluR-K2; glutamate receptor; Glutamate receptor 2; glutamate receptor B; glutamate receptor B flip isoform; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, AMPA 2; OTTHUMP00000165324; OTTHUMP00000220942; OTTHUMP00000220943

基因别名: GluA2; Glur-2; GluR-B; GluR-K2; GLUR2; GLURB; GRIA2; HBGR2

UniProt ID: (Human) P42262, (Rat) P19491

Entrez Gene ID: (Human) 2891, (Mouse) 14800, (Rat) 29627

分子生物学功能: transmembrane signal receptor