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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Glucocorticoid Receptor (Nr3c1) Antibodies

          Invitrogen

          Glucocorticoid Receptor alpha Polyclonal Antibody

          高级验证
          2个已发表图片
          40 篇参考文献
          查看其他30个Glucocorticoid Receptor (Nr3c1)抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite Glucocorticoid Receptor alpha Polyclonal Antibody

          • 抗体检测数据 (6)
          • 已发表图片 (2)
          • 高级验证 (1)
          Glucocorticoid Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Glucocorticoid Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 9

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          Glucocorticoid Receptor alpha Antibody (PA1-516) in ICC/IF

          Immunofluorescent analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody (Product # PA1-516) shows staining in A2058 Cells. Glucocorticoid Receptor alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Glucocorticoid Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
          Glucocorticoid Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
          Glucocorticoid Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
          Glucocorticoid Receptor alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Glucocorticoid Receptor alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Glucocorticoid Receptor alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Glucocorticoid Receptor alpha Antibody in Immunocytochemistry (ICC/IF)
          Glucocorticoid Receptor alpha Antibody in ChIP Assay (ChIP)
          Glucocorticoid Receptor alpha Antibody

          产品信息

          PA1-516

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          4 µg/mL
          查看17篇已发表文章 17篇已发表文章

          免疫组化 (IHC)

          -
          查看3篇已发表文章 3篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:20
          -

          免疫细胞化学 (ICC/IF)

          Assay-dependent
          查看8篇已发表文章 8篇已发表文章

          流式细胞分析 (Flow)

          -
          查看2篇已发表文章 2篇已发表文章

          免疫沉淀 (IP)

          Assay-dependent
          查看1篇已发表文章 1篇已发表文章

          染色质免疫沉淀 实验 (ChIP)

          Assay-dependent
          查看7篇已发表文章 7篇已发表文章

          凝胶阻滞分析 (GS)

          -
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Human, Mouse, Rat

          已发表种属

          Human, Mouse, Non-human primate, Plant, Rat, Sheep

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic Peptide: C(755) E I I T N Q I P K Y S N G N I K K(771)
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          浓度

          1 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2251653

          产品详细信息

          PA1-516 detects glucocorticoid receptor (GR) alpha from human, mouse and rat tissues. This antibody does not detect GR beta.

          PA1-516 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunoprecipitation (ChIP) procedures. By Western blot, this antibody detects an ~94 kDa protein representing GR in HeLa cell lysate.

          PA1-516 immunizing peptide corresponds to amino acid residues 755-771 of human GR alpha. This peptide (Cat. # PEP-221) is available for use in neutralization and control experiments.

          Reconstitute with 50 µL of distilled water.

          靶标信息

          Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858

          查看更多 收起

          基因别名: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1

          查看更多 收起

          UniProt ID: (Human) P04150, (Mouse) P06537, (Rat) P06536

          查看更多 收起

          Entrez Gene ID: (Human) 2908, (Mouse) 14815, (Rat) 24413

          查看更多 收起

          功能
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding glucocorticoid receptor activity steroid binding protein binding zinc ion binding protein complex binding glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity steroid hormone binding RNA polymerase II regulatory region DNA binding DNA binding chromatin binding double-stranded DNA binding steroid hormone receptor activity transcription coactivator activity transcription factor binding lipid binding Hsp70 protein binding receptor tyrosine kinase binding heat shock protein binding hormone binding protein homodimerization activity sequence-specific DNA binding metal ion binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding
          参与通路
          regulation of gluconeogenesis chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glucocorticoid metabolic process regulation of glucose metabolic process positive regulation of glutamate secretion chromatin modification adrenal gland development negative regulation of synaptic plasticity regulation of glucocorticoid biosynthetic process regulation of cell proliferation maternal behavior glucocorticoid receptor signaling pathway negative regulation of vascular permeability steroid hormone mediated signaling pathway glucocorticoid mediated signaling pathway positive regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter response to arsenic-containing substance chromatin-mediated maintenance of transcription mammary gland duct morphogenesis positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development cellular response to steroid hormone stimulus cellular response to dexamethasone stimulus negative regulation of glucocorticoid mediated signaling pathway positive regulation of glucocorticoid receptor signaling pathway brain development aging circadian rhythm androgen metabolic process response to radiation response to activity response to inactivity muscle atrophy lung development response to insulin response to mercury ion response to corticosterone response to calcium ion response to electrical stimulus cellular response to magnesium ion response to dexamethasone transcription initiation from RNA polymerase II promoter apoptotic process chromosome segregation mitotic nuclear division signal transduction covalent chromatin modification cell division
          It has to be done as per old AB suggested Products section.
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