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Invitrogen
Glucocorticoid Receptor beta Polyclonal Antibody
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产品信息
PA3-514
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse,
Pig,
Sheep
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic Peptide: N(728) V M W L K P E S T S H T L I(742) C
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
保存液
whole serum, PBS
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA3-514 detects glucocorticoid receptor (GR) beta from human cells.
PA3-514 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~90 kDa protein representing GR beta from HeLa cell lysate. Immunocytochemical staining of GR beta in HeLa cells with PA3-514 results in nuclear staining.
PA3-514 immunizing peptide corresponds to amino acid residues 728-742 from human GR beta. This peptide (Cat. # PEP-222) is available for use in neutralization and control experiments.
靶标信息
Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR beta might function as a dominant negative inhibitor of GR alpha activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Glucocorticoid receptor; GR; GR Beta; hGR DL-1; hGR NS-1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3 group C member 1 variant hGR-B(54); nuclear receptor subfamily 3 group C member 1 variant hGR-B(77); nuclear receptor subfamily 3 group C member 1 variant hGR-B(93); nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); putative upstream ORF.; Unknown; unnamed protein product
基因别名: GCCR; GCR; GCRST; GR; GRL; NR3C1
UniProt ID: (Human) P04150
Entrez Gene ID: (Human) 2908
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
glucocorticoid receptor activity
steroid binding
protein binding
zinc ion binding
lipid binding
TBP-class protein binding
protein kinase binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
metal ion binding
Hsp90 protein binding
steroid hormone binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
regulation of gluconeogenesis
chromatin organization
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
chromosome segregation
signal transduction
glucocorticoid metabolic process
response to wounding
gene expression
microglia differentiation
adrenal gland development
intracellular steroid hormone receptor signaling pathway
regulation of glucocorticoid biosynthetic process
nuclear receptor-mediated corticosteroid signaling pathway
synaptic transmission, glutamatergic
maternal behavior
glucocorticoid receptor signaling pathway
positive regulation of neuron apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
astrocyte differentiation
cell division
response to glucocorticoid
response to cortisol
mammary gland duct morphogenesis
motor behavior
cellular response to steroid hormone stimulus
cellular response to glucocorticoid stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
neuroinflammatory response
response to ketone
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
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