Invitrogen

Granzyme B Monoclonal Antibody (NGZB), eFluor™ 660, eBioscience™

Granzyme B Antibody in Flow Cytometry (Flow)

图: 1 / 45

Granzyme B Antibody (50-8898-82) in Flow

C57Bl/6 splenocytes were stimulated for 4 days with Anti-Mouse CD3e and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 & Product # 16-0281-82) and Brefeldin A was added for the last 5 hours of culture. Cells were surface stained with Anti-Mouse CD8a FITC (Product # 11-0081-82) followed by intracellular staining with 0.06 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.06 µg of Anti-Mouse Granzyme B eFluor® 660 (right) using the Intracellular Fixation & Permeabilization Buffer Set (plus Bref... View More

Granzyme B Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

50-8898-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看45篇已发表文章 45篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

NGZB

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11219679

产品详细信息

Description: This NGZB monoclonal antibody reacts with mouse Granzyme B, which is a member of the granzyme serine protease family. Granzyme B is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. This NGZB monoclonal antibody does not cross-react to human Granzyme B nor is staining blocked with GB11, suggesting it recognizes a different epitope.

Applications Reported: This NGZB antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This NGZB antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

KEAP1 promotes anti-tumor immunity by inhibiting PD-L1 expression in NSCLC.

Cell death & disease

Li J,Shi D,Li S,Shi X,Liu Y,Zhang Y,Wang G,Zhang C,Xia T,Piao HL,Liu HX

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Feb 27 00:00:00 UTC 2024

Inhibition of choline metabolism in an angioimmunoblastic T-cell lymphoma preclinical model reveals a new metabolic vulnerability as possible target for treatment.

Journal of experimental & clinical cancer research : CR

Krug A,Tosolini M,Madji Hounoum B,Fournié JJ,Geiger R,Pecoraro M,Emond P,Gaulard P,Lemonnier F,Ricci JE,Verhoeyen E

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Feb 06 00:00:00 UTC 2024

68Ga-FAPI PET imaging monitors response to combined TGF-βR inhibition and immunotherapy in metastatic colorectal cancer.

The Journal of clinical investigation

Li K,Liu W,Yu H,Chen J,Tang W,Wang J,Qi M,Sun Y,Xu X,Zhang J,Li X,Guo W,Li X,Song S,Tang S

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Thu Jan 04 00:00:00 UTC 2024

Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy.

Nature communications

Wang Z,Li W,Jiang Y,Tran TB,Cordova LE,Chung J,Kim M,Wondrak G,Erdrich J,Lu J

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Thu Nov 09 00:00:00 UTC 2023

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri May 19 00:00:00 UTC 2023

Small-molecule PTPN2 Inhibitors Sensitize Resistant Melanoma to Anti-PD-1 Immunotherapy.

Cancer research communications

Zhu Z,Tang R,Huff S,Kummetha IR,Wang L,Li N,Rana TM

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Sun Jan 01 00:00:00 UTC 2023

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2022

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2022

Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis.

Frontiers in immunology

Maity S,Chakraborty A,Mahata SK,Roy S,Das AK,Sen M

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Mar 22 00:00:00 UTC 2022

Intranasal Delivery of Recombinant S100A8 Protein Delays Lung Cancer Growth by Remodeling the Lung Immune Microenvironment.

Frontiers in immunology

Wong SW,McCarroll J,Hsu K,Geczy CL,Tedla N

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Jun 06 00:00:00 UTC 2022

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.

Journal for immunotherapy of cancer

Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Immunohistochemistry

Tue Mar 01 00:00:00 UTC 2022

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.

Journal for immunotherapy of cancer

Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Immunohistochemistry

Tue Mar 01 00:00:00 UTC 2022

RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer.

The Journal of clinical investigation

Deng P,Wang Z,Chen J,Liu S,Yao X,Liu S,Liu L,Yu Z,Huang Y,Xiong Z,Xiao R,Gao J,Liang W,Chen J,Liu H,Hong JH,Chan JY,Guan P,Chen J,Wang Y,Yin J,Li J,Zheng M,Zhang C,Zhou P,Kang T,Teh BT,Yu Q,Zuo Z,Jiang Q,Liu J,Xiong Y,Xia X,Tan J

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Nov 15 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Dec 12 00:00:00 UTC 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

Camptothesome elicits immunogenic cell death to boost colorectal cancer immune checkpoint blockade.

Journal of controlled release : official journal of the Controlled Release Society

Wang Z,Li W,Park J,Gonzalez KM,Scott AJ,Lu J

50-8898-82 was used in Flow cytometry/Cell sorting to decipher that Camptothesome was taken up by colorectal cancer (CRC) cells through primarily the clathrin-mediated endocytotic pathway and displayed the potential of eliciting robust immunogenic cancer cell death (ICD) via upregulating calreticulin, high mobility group box 1 protein (HMGB-1), and adenosine triphosphate (ATP), three hallmarks involved in the induction of ICD.

Thu Sep 01 00:00:00 UTC 2022

Non-synergy of PD-1 blockade with T-cell therapy in solid tumors.

Journal for immunotherapy of cancer

Davies JS,Karimipour F,Zhang L,Nagarsheth N,Norberg S,Serna C,Strauss J,Chiou S,Gulley JL,Hinrichs CS

50-8898-82 was used in Flow cytometry/Cell sorting to support the concept that PD-1 blockade acts primarily through endogenous rather than transferred T cells to mediate a non-synergistic antitumor effect in solid tumor cell therapy.

Fri Jul 01 00:00:00 UTC 2022

Blockade of 6-phosphogluconate dehydrogenase generates CD8⁺ effector T cells with enhanced anti-tumor function.

Cell reports

Daneshmandi S,Cassel T,Lin P,Higashi RM,Wulf GM,Boussiotis VA,Fan TW,Seth P

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Mar 09 00:00:00 UTC 2021

Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy.

Cell reports

Dodagatta-Marri E,Ma HY,Liang B,Li J,Meyer DS,Chen SY,Sun KH,Ren X,Zivak B,Rosenblum MD,Headley MB,Pinzas L,Reed NI,Del Cid JS,Hann BC,Yang S,Giddabasappa A,Noorbehesht K,Yang B,Dal Porto J,Tsukui T,Niessen K,Atakilit A,Akhurst RJ,Sheppard D

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Jul 06 00:00:00 UTC 2021

Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

Cell communication and signaling : CCS

Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Aug 30 00:00:00 UTC 2021

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8⁺ T Cells and Macrophages.

International journal of nanomedicine

Luo ZW,Xia K,Liu YW,Liu JH,Rao SS,Hu XK,Chen CY,Xu R,Wang ZX,Xie H

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection.

iScience

Steele L,Mannion AJ,Shaw G,Maclennan KA,Cook GP,Rudd CE,Taylor A

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri Jun 25 00:00:00 UTC 2021

Circadian Clock Disruption Suppresses PDL1⁺ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer.

Cellular and molecular gastroenterology and hepatology

Liu JL,Wang CY,Cheng TY,Rixiati Y,Ji C,Deng M,Yao S,Yuan LH,Zhao YY,Shen T,Li JM

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Jan 24 00:00:00 UTC 2022

Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.

NPJ precision oncology

Yang N,Ji F,Cheng L,Lu J,Sun X,Lin X,Lan X

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri May 07 00:00:00 UTC 2021

Identification of HLA-A2-Restricted Mutant Epitopes from Neoantigens of Esophageal Squamous Cell Carcinoma.

Vaccines

Wang Z,Ran L,Chen C,Shi R,Dong Y,Li Y,Zhou X,Qi Y,Zhu P,Gao Y,Wu Y

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Protein Assays and Analysis

Fri Oct 01 00:00:00 UTC 2021

Glutathione Restricts Serine Metabolism to Preserve Regulatory T Cell Function.

Cell metabolism

Kurniawan H,Franchina DG,Guerra L,Bonetti L,-Baguet LS,Grusdat M,Schlicker L,Hunewald O,Dostert C,Merz MP,Binsfeld C,Duncan GS,Farinelle S,Nonnenmacher Y,Haight J,Das Gupta D,Ewen A,Taskesen R,Halder R,Chen Y,Jäger C,Ollert M,Wilmes P,Vasiliou V,Harris IS,Knobbe-Thomsen CB,Turner JD,Mak TW,Lohoff M,Meiser J,Hiller K,Brenner D

50-8898 was used in Flow cytometry/Cell sorting to reveal an unexpected role for GSH in restricting serine availability to preserve Treg functionality.

Tue May 05 00:00:00 UTC 2020

Assessment of the WAP-Myc mouse mammary tumor model for spontaneous metastasis.

Scientific reports

Utz B,Turpin R,Lampe J,Pouwels J,Klefström J

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri Oct 30 00:00:00 UTC 2020

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Wed Dec 16 00:00:00 UTC 2020

Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B.

PloS one

Parra M,Weitner M,Yang A,Akue A,Liu X,Schmidt T,Allman WR,Akkoyunlu M,Derrick SC

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Oct 27 00:00:00 UTC 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Thu Nov 28 00:00:00 UTC 2019

Myeloid Cells Restrict MCMV and Drive Stress-Induced Extramedullary Hematopoiesis through STAT1.

Cell reports

Gawish R,Bulat T,Biaggio M,Lassnig C,Bago-Horvath Z,Macho-Maschler S,Poelzl A,Simonović N,Prchal-Murphy M,Rom R,Amenitsch L,Ferrarese L,Kornhoff J,Lederer T,Svinka J,Eferl R,Bosmann M,Kalinke U,Stoiber D,Sexl V,Krmpotić A,Jonjić S,Müller M,Strobl B

50-8898 was used in Flow cytometry/Cell sorting to provide genetic evidence that signaling through STAT1 in myeloid cells is required to restrict MCMV at early time points post-infection and to induce compensatory hematopoiesis in the spleen.

Tue Feb 26 00:00:00 UTC 2019

Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8⁺ T Cells.

Immunity

Li J,Lee Y,Li Y,Jiang Y,Lu H,Zang W,Zhao X,Liu L,Chen Y,Tan H,Yang Z,Zhang MQ,Mak TW,Ni L,Dong C

50-8898 was used in Flow cytometry/Cell sorting to demonstrate that increased B7S1 expression in myeloid cells from human hepatocellular carcinoma is correlated with CD8+ T cell dysfunction.

Tue Apr 17 00:00:00 UTC 2018

Breast Cancer Chemo-immunotherapy through Liposomal Delivery of an Immunogenic Cell Death Stimulus Plus Interference in the IDO-1 Pathway.

ACS nano

Lu J,Liu X,Liao YP,Wang X,Ahmed A,Jiang W,Ji Y,Meng H,Nel AE

50-8898 was used in Flow cytometry/Cell sorting to develop a chemo-immunotherapy approach to reduce the high mortality of metastatic breast cancer, utilising a liposomal carrier to trigger immunogenic cell death.

Tue Nov 27 00:00:00 UTC 2018

MAIT cells contribute to protection against lethal influenza infection in vivo.

Nature communications

van Wilgenburg B,Loh L,Chen Z,Pediongco TJ,Wang H,Shi M,Zhao Z,Koutsakos M,Nüssing S,Sant S,Wang Z,D'Souza C,Jia X,Almeida CF,Kostenko L,Eckle SBG,Meehan BS,Kallies A,Godfrey DI,Reading PC,Corbett AJ,McCluskey J,Klenerman P,Kedzierska K,Hinks TSC

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri Nov 09 00:00:00 UTC 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Nov 19 00:00:00 UTC 2018

Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.

Nature communications

Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Feb 05 00:00:00 UTC 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

50-8898 was used in Flow cytometry/Cell sorting to show that miR-27 has a role in regulating Treg immune cells and that correct control of miR-27 expression is important to safeguard immunological tolerance..

Wed Feb 01 00:00:00 UTC 2017

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Wed Apr 12 00:00:00 UTC 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Mon Jun 05 00:00:00 UTC 2017

1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8⁺ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model.

Frontiers in immunology

Dai K,Huang L,Huang YB,Chen ZB,Yang LH,Jiang YA

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Fri Nov 17 00:00:00 UTC 2017

IL-12 and IL-4 activate a CD39-dependent intrinsic peripheral tolerance mechanism in CD8(+) T cells.

European journal of immunology

Noble A,Mehta H,Lovell A,Papaioannou E,Fairbanks L

50-8898 was used in Flow cytometry/Cell sorting to study the upregulation of CD39 on CD8(+) T cells and identify a novel pathway of peripheral tolerance.

Wed Jun 01 00:00:00 UTC 2016

Protein Translation Activity: A New Measure of Host Immune Cell Activation.

Journal of immunology (Baltimore, Md. : 1950)

Seedhom MO,Hickman HD,Wei J,David A,Yewdell JW

50-8898 was used in Flow cytometry/Cell sorting to describe the in vivo ribopuromycylation method.

Mon Aug 15 00:00:00 UTC 2016

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.

Journal of virology

Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.

Journal of virology

Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Non-redundant requirement for CXCR3 signalling during tumoricidal T-cell trafficking across tumour vascular checkpoints.

Nature communications

Mikucki ME,Fisher DT,Matsuzaki J,Skitzki JJ,Gaulin NB,Muhitch JB,Ku AW,Frelinger JG,Odunsi K,Gajewski TF,Luster AD,Evans SS

50-8898 was used in Flow cytometry/Cell sorting to investigate the hierarchy of chemokine receptor requirements during T-cell trafficking to murine and human melanoma.

Thu Jun 25 00:00:00 UTC 2015

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using Granzyme B monoclonal antibody (Product # 50-8898-82) in Flow Cytometry

Tue Oct 28 00:00:00 UTC 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

50-8898 was used in Flow cytometry/Cell sorting to study the role of Tim-3 in the regulation of natural killer cells following LPS-induced shock.

Mon Jul 06 00:00:00 UTC 2015

生物信息学

蛋白别名: CCP1; CTLA-1; Cytotoxic cell protease 1; Cytotoxic T lymphocyte associated serine esterase 1; Fragmentin-2; Granzyme B(G,H); GranzymeB; Human lymphocyte protein (Hlp); OTTHUMP00000028189

查看更多收起

基因别名: AI553453; CCP-1/C11; CCP1; Ctla-1; Ctla1; GZB; Gzmb

查看更多收起

UniProt ID: (Mouse) P04187

查看更多收起

Entrez Gene ID: (Mouse) 14939

查看更多收起

功能

serine-type endopeptidase activity protein binding peptidase activity serine-type peptidase activity hydrolase activity protease serine protease protein modifying enzyme

参与通路

T cell mediated cytotoxicity proteolysis apoptotic process granzyme-mediated apoptotic signaling pathway protein processing cytolysis