Invitrogen

H-Ras Polyclonal Antibody

Name: H-Ras Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-22392

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查看其他23个H-Ras抗体

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说明书

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技术支持

说明书

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H-Ras Antibody in Immunocytochemistry (ICC/IF)

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H-Ras Antibody (PA5-22392) in ICC/IF

Immunofluorescent analysis of H-Ras in paraformaldehyde-fixed HeLa cells using a H-Ras polyclonal antibody (Product # PA5-22392) at a 1:200 dilution.

H-Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

H-Ras Antibody — Figure 2 DUF5 Vv is a Ras site-specific endopeptidase. ( a ) Coomassie-stained 18% SDS-polyacrylamide gel of anti-HA immunoprecipitated proteins from cells expressing HA-HRas treated for 24 h as indicated. Lower band (HRas*) was excised for peptide sequencing with HRas peptide coverage highlighted in yellow. ( b ) Same fractions probed by immunoblotting to detect the N terminus (anti-HA) and C terminus (isotype-specific antibody). ( c ) Lysates from cells expressing HA-tagged KRas, NRas or HRas probed by immunoblotting as indicated. ( d ) In-vitro cleavage of 10 muM rKRas to KRas* with 10 muM rDUF5 Vv (inset) or concentration indicated. Error bars indicate mean+-s.d. ( n =3). ( e ) In-vitro cleavage of 10 muM rKRas, rHRas and rNRas with 10 muM rDUF5 Vv . Identical results of Edman degradation were obtained for all three proteins. ( f ). Black arrow indicates the cleavage site in the Switch I region (red) of HRas 69 . {TM}

产品信息

PA5-22392

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

Assay-dependent

产品规格

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 111 and 189 of H-Ras (Uniprot ID#P01112)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.47 mg/mL

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11152295

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat2, HRAS-transfected 293T, RFL6, PLC5Rat2, RFL6, PLC5.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways leading to distinct cytoplasmic or nuclear events. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K-, N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibition of apoptosis. K-Ras mutations are common in pancreatic, colorectal and nonsmall-cell lung carcinomas; H-Ras mutations are common in bladder, kidney and thyroid carcinomas; N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinaoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

H-Ras Polyclonal Antibody

H-Ras Antibody (PA5-22392) in ICC/IF

产品信息

PA5-22392

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

保存条件

运输条件

RRID

产品详细信息

靶标信息

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