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HDAC4 Antibody (711390) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized HeLa cells for detection of endogenous HDAC4 using Anti-HDAC4 Recombinant Rabbit Polyclonal Antibody (Product # 711390, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HDAC4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product ... View More
产品信息
711390
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Polyclonal
类型
Antibody
克隆号
14HCLC
抗原
Peptides corresponding to Human HDAC4 (aa 568-585, 147-163)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633171
产品详细信息
This antibody is predicted to react with Monkey, Rat, Pig and Bovine.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
Histone deacetylase 4 (HDAC4) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is also involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. It is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Mutations to the HDAC4 gene have been found in Brachydactyly-mental retardation syndrome (BDMR).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp779K1053; HD4; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 4; histone deacetylase A; KIAA0744; MITR
基因别名: AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDAC4; HDACA; KIAA0288
UniProt ID: (Human) P56524
Entrez Gene ID: (Human) 9759
RNA polymerase III transcription factor binding
core promoter binding
chromatin binding
transcription corepressor activity
histone deacetylase activity
protein binding
transcription factor binding
zinc ion binding
protein kinase binding
potassium ion binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
activating transcription factor binding
histone deacetylase binding
sequence-specific DNA binding
transcription regulatory region DNA binding
repressing transcription factor binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
osteoblast development
chromatin remodeling
transcription, DNA-templated
inflammatory response
nervous system development
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of lamellipodium assembly
negative regulation of myotube differentiation
regulation of cardiac muscle contraction by calcium ion signaling
response to denervation involved in regulation of muscle adaptation
cardiac muscle hypertrophy in response to stress
positive regulation of smooth muscle cell migration
chromatin modification
histone deacetylation
B cell differentiation
positive regulation of protein sumoylation
peptidyl-lysine deacetylation
regulation of gene expression, epigenetic
B cell activation
response to drug
regulation of protein binding
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of neuron apoptotic process
negative regulation of osteoblast differentiation
negative regulation of glycolytic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
regulation of skeletal muscle fiber development
positive regulation of sequence-specific DNA binding transcription factor activity
response to interleukin-1
histone H3 deacetylation
histone H4 deacetylation
cellular response to mechanical stimulus
cellular response to tumor necrosis factor
cellular response to parathyroid hormone stimulus
positive regulation of reactive oxygen species biosynthetic process
