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Invitrogen
HIF-2 alpha Polyclonal Antibody
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HIF-2 alpha Antibody (PA5-19494) in ICC/IF
Immunofluorescent staining of Rat PC12 cells using Product # PA5-19494, anti-HIF2 alpha antibody. The cells were fixed with PFA (4%)for 10 minutes, permabilised PBS-T for 20 minutes and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. A solution of BSA (1%), normal goat serum (10%) and glycine (0.3 M) was used to quench autofluorescence and block non-specific protein-protein in... View More
产品信息
PA5-19494
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to Mouse HIF2-alpha aa 600-700 (internal sequence) KLH
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
For Western Blot, this antibody detects a HIF-2-alpha specific band at ~96 kDa and has non-specific bands at 240 kDa,25 kDa and 45 kDa.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with mouse based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
靶标信息
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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