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HLA-DRB1 Antibody (PA5-95600) in WB
Western blot analysis of HLA-DR/DP in Lane 1: human Raji whole cell lysate, Lane 2: human Raji whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with HLA-DR/DP polyclonal antibody (Product # PA5-95600) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemilum... View More
Product Details
PA5-95600
产品规格
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human HLA Class II DRB1/HLA-DRB1 (TERVQFLERLFYNQEEFVRFDSDVGEYRAVTELGR).
Conjugate
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2807402
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: DW2.2/DR2.2; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA-DRB1; human leucocyte antigen DRB1; Human leukocyte antigen DRB1; lymphocyte antigen DRB1; MHC class II antigen; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta
Gene Aliases: DRB1; DRw10; HLA-DR1B; HLA-DRB; HLA-DRB1; SS1
UniProt ID: (Human) Q4PRC3
Entrez Gene ID: (Human) 3123
Molecular Function:
major histocompatibility complex protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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