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Invitrogen
HNF4A Polyclonal Antibody
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HNF4A Antibody (PA5-142255) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of HNF4A in NIH3T3 cells using HNF4A Polyclonal Antibody (Product # PA5-142255). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing a low level of nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-142255
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence C-HPHLMQEHMGTN (aa 390-401).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Ammonium sulfate precipitation, Antigen affinity chromatography
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Additional Information: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
靶标信息
Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ39654; HNF4 alpha; HNF4-alpha; Nuclear receptor 2A1; nuclear receptor subfamily 2 group A member 1; transcription factor; transcription factor 14; transcription factor HNF-4; unnamed protein product
基因别名: HNF-4; Hnf4; Hnf4alpha; MODY1; Nr2a1; TCF-14; Tcf14
UniProt ID: (Mouse) P49698
Entrez Gene ID: (Mouse) 15378
fatty-acyl-CoA binding
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription cofactor binding
transcription corepressor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
receptor binding
fatty acid binding
protein binding
zinc ion binding
protein domain specific binding
long-chain fatty acyl-CoA binding
protein homodimerization activity
sequence-specific DNA binding
macromolecular complex binding
arachidonic acid binding
long-chain acyl-CoA hydrolase activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
stearic acid binding
sequence-specific double-stranded DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
lipid metabolic process
acyl-CoA metabolic process
xenobiotic metabolic process
establishment of tissue polarity
sex differentiation
blood coagulation
negative regulation of cell proliferation
response to glucose
gene expression
regulation of gastrulation
positive regulation of gene expression
regulation of lipid metabolic process
signal transduction involved in regulation of gene expression
cell differentiation
negative regulation of cell growth
negative regulation of cell migration
intracellular receptor signaling pathway
regulation of microvillus assembly
response to trichostatin A
negative regulation of protein import into nucleus
glucose homeostasis
cholesterol homeostasis
regulation of circadian rhythm
cell-cell junction organization
positive regulation of gluconeogenesis
positive regulation of fatty acid biosynthetic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
positive regulation of transcription from RNA polymerase II promoter
regulation of insulin secretion
lipid homeostasis
phospholipid homeostasis
negative regulation of protein tyrosine kinase activity
triglyceride homeostasis
positive regulation of ornithine catabolic process
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