Invitrogen

HOXA5 Polyclonal Antibody

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HOXA5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HoxA5 (red) in HUES3::Hb9-GFP human embryonic stem cells patterned to cervical neuromesodermal progenitors as previously described (Lippmann et. al. 2015) and subsequently differentiated into motor neurons (green) using smoothened agonist, purmorphamine and retinoic acid. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 5% normal donkey serum in tris buffered saline containing 0.3% Triton-X (TBSDT) for at least one hour at 4C. Cells were stained with a monoclonal HoxA5 antibody (Product # PA5-50045; 1:100) and GFP antibody in TBSDT overnight at 4C, washed, and then incubated with an Alexa Fluor secondary antibodies (1:500) for one hour at room temperature. Nuclei (blue) were counterstained with DAPI. Images were taken on a Nikon AR1 confocal microscope at 40X magnification. Data courtesy of Dr Ashton's lab at University of Wisconsin at Madison.

产品信息

PA5-50045

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	-
免疫细胞化学 (ICC/IF)	1:100	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthesized peptide derived from N-terminal of human HXA5. 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20°C
RRID	AB_2635498

产品详细信息

The antibody detects endogenous levels of total HOXA5 protein.

This antibody detects HoxA5 in cervical neuromesodermal progenitors.

靶标信息

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA5 gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: homeo box 1C; homeo box A5; Homeobox protein Hox-1.3; Homeobox protein Hox-1C; Homeobox protein Hox-A5; homeobox protein HOXA5; Homeobox protein M2; hox-1.3; Hox-1C

基因别名: Hox-1.3; HOX1; HOX1.3; HOX1C; Hoxa-5; HOXA5

UniProt ID: (Human) P20719, (Mouse) P09021

Entrez Gene ID: (Human) 3202, (Rat) 79241, (Mouse) 15402

分子生物学功能: homeodomain transcription factor