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Active Motif
HP1 alpha Recombinant Mouse Monoclonal Antibody, AbFlex®
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产品信息
91405ACTMOTIF
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG2a
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
抗原
This HP1 alpha antibody was raised against a recombinant protein corresponding to full-length recombinant mouse HP1 alpha.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
32mM sodium acetate/140mM HEPES, pH 7.5, with 30% glycerol, 70mM NaCl
内含物
0.035% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® HP1α antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HP1 (Heterochromatin protein 1) is a chromosomal protein that participates in both chromatin packaging and gene silencing. Three HP1 isoforms (α, β and γ) occur in mammals. The chromodomain (Chromatin Organization Modifier) of HP1α is responsible for binding to Histone H3 methyl Lys9, although additional factors are required to specify the HP1 targeting. The chromoshadow domain mediates interactions with a number of nuclear proteins, including SuvH39 (a Histone H3 Lysine methyltransferase), the DNA methyltransferases DNMT1 and DNMT3A or the large subunit of the chromatin assembly factor-1 (CAF-1). Dysfunction of HP1α plays a critical role in tumorigenesis, and is implicated in the invasive/metastatic phenotype of breast cancer.
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
靶标信息
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Antigen p25; Cbx5 antibody; chromobox homolog 5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; heterochromatin protein 1 alpha; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; mHP1(alpha)
基因别名: 2610029O15Rik; C75991; CBX5; HEL25; HP1; HP1A; Hp1alpha
UniProt ID: (Human) P45973, (Mouse) Q61686
Entrez Gene ID: (Human) 23468, (Mouse) 12419
