Invitrogen

HP1 beta Monoclonal Antibody (4E12)

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HP1 beta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HP1 was performed using MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HP1 beta Monoclonal Antibody (Product # MA5-21203) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear localization of HP1. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

HP1 beta Antibody in Western Blot (WB) — Western blot was performed using Anti-HP1 beta Monoclonal Antibody (Product # MA5-21203) and a 25kDa band corresponding to HP1 was observed across the cell lines and tissues tested. Modified whole cell extracts (30ug) of HeLa (Lane 1), MCF7 (Lane 2), A-431 (Lane 3), U-2 OS (Lane 4), PC-3 (Lane 5), Raji (Lane 6), IMR32 (Lane 7), Hep G2 (Lane 8), Mouse Liver (Lane 9) and Rat Liver (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HP1 beta Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged CBX1 using anti-CBX1 monoclonal antibody (Product # MA5-21203) is 0.1 ng/mL as a capture antibody.

产品信息

MA5-21203

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

免疫细胞化学 (ICC/IF)

5-10 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human, Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4E12

抗原

CBX1 (NP_006798.1, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608236

产品详细信息

Peptide Sequence: GKKQNKKKVE EVLEEEEEEY VVEKVLDRRV VKGKVEYLLK WKGFSDEDNT WEPEENLDCP DLIAEFLQSQ KTAHETDKSE GGKRKADSDS EDKGEESK

靶标信息

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: chromobox homolog 1; chromobox homolog 1 (HP1 beta homolog Drosophila ); Chromobox protein homolog 1; Heterochromatin protein 1 homolog beta; heterochromatin protein 1-beta; Heterochromatin protein p25; heterochromatin protein p25 beta; HGNC:1551; HP1 beta; HP1 beta homolog; HP1Hsbeta; M31; Modifier 1 protein; p25beta

基因别名: CBX; CBX1; HP1-BETA; HP1Hs-beta; HP1Hsbeta; M31; MOD1; p25beta

UniProt ID: (Human) P83916

Entrez Gene ID: (Human) 10951, (Rat) 360609

功能

negative regulation of transcription, DNA-templated

参与通路

chromatin binding protein binding enzyme binding protein homodimerization activity histone methyltransferase binding identical protein binding