Invitrogen

HSF2 Polyclonal Antibody

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HSF2 Antibody (PA5-96200) in WB

Western blot analysis of HSF2 in various lysates. Samples were incubated with HSF2 Polyclonal antibody (Product # PA5-96200) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-96200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 39-215 of human HSF2 (Q03933)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.46 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2808002

产品详细信息

Positive Samples: 293T, HepG2, SW620, BT-474; Cellular Location: Cytoplasm, Nucleus

Immunogen sequence: LDEQRFAKEI LPKYFKHNNM ASFVRQLNMY GFRKVVHIDS GIVKQERDGP VEFQHPYFKQ GQDDLLENIK RKVSSSKPEE NKIRQEDLTK IISSAQKVQI KQETIESRLS ELKSENESLW KEVSELRAKH AQQQQVIRKI VQFIVTLVQN NQLVSLKRKR PLLLNTNGAQ KKNLFQH

靶标信息

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。