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FIGURE: 1 / 1
HSF2 Antibody (PA5-96200) in WB
Western blot analysis of HSF2 in various lysates. Samples were incubated with HSF2 Polyclonal antibody (Product # PA5-96200) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96200
产品规格
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
A recombinant fusion protein containing a sequence corresponding to amino acids 39-215 of human HSF2.
Conjugate
Unconjugated
形式
Liquid
浓度
0.46 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2808002
Product Specific Information
Positive Samples: 293T, HepG2, SW620, BT-474; Cellular Location: Cytoplasm, Nucleus
Target Information
Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: heat shock factor 2; Heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; HSTF 2; MGC117376; MGC156196; MGC75048
Gene Aliases: HSF 2; HSF2; HSTF 2; HSTF2
UniProt ID: (Human) Q03933
Entrez Gene ID: (Human) 3298, (Rat) 64441
Molecular Function:
winged helix/forkhead transcription factor
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
protein homodimerization activity
DNA binding
sequence-specific DNA binding
Performance Guarantee
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