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Invitrogen
HSP90 Monoclonal Antibody (8D3), APC
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HSP90 Antibody (MA5-45106) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Hsp90 complex monoclonal antibody (Product # MA5-45106) at 1:100 for 12 hours at 4°C, followed by R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex antibody. (C) Composite.
产品信息
MA5-45106
产品规格
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
8D3
抗原
Ah receptor (Aryl hydrocarbon receptor)
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. MA5-45106 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop. |Detects 90kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha ) kinase (HRI).
靶标信息
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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