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Invitrogen
HSP90 alpha Monoclonal Antibody (3G3)
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HSP90 alpha Antibody (MA3-011) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 90 using Heat Shock Protein 90 Monoclonal antibody (3G3) (Product # MA3-011) shows staining in WiDr colon carcinoma cells. Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or ... View More
产品信息
MA3-011
应用
建议稀释比
已发表文章
产品规格
已发表种属
Fruit fly,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat,
Virus
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
3G3
抗原
Purified mouse HSP90.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-011 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, rainbow trout, and chicken tissues.
MA3-011 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from Hepa 1 cell extract. It has been reported that up to 90% of cytosolic HSP90 can be immunoprecipitated with this product under low ionic strength conditions.
For Western blots using MA3-011, nitrocellulose is recommended. PVDF membranes typically do not yield acceptable results with this antibody.
Before use, centrifuge suspension (4000 g for 15 minutes) and discard supernatant. Resuspend pellet in 200 µL of PBS, pH 7.2. If desired, 0.05% sodium azide may be added to resuspended solution. Removal of residual ammonium sulfate is not usually necessary for immunoprecipitation and Western blot procedures, however, any other uses may require its removal by dialysis or equivalent method. Stable for 6 months after PBS resuspension.
The MA3-011 antigen is purified HSP90 from Hepa 1 cytosol.
靶标信息
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
