Invitrogen

HUNK Polyclonal Antibody

Name: HUNK Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-28765

高级验证

4个已发表图片

4 篇参考文献

查看其他15个HUNK抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

HUNK Antibody in Immunocytochemistry (ICC/IF)

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HUNK Antibody (PA5-28765) in ICC/IF

Immunocytochemistry analysis of HUNK in paraformaldehyde-fixed A549. Samples were incubated with HUNK polyclonal antibody (Product # PA5-28765) using a dilution of 1:200.

HUNK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HUNK Antibody in Immunoprecipitation (IP)

Product Image Gallery

HUNK Antibody — STU has similar activity in cells as HUNK inhibition (A) Control and HUNK knockdown SMF cells expressing a control shRNA or a shRNA targeting mouse HUNK (Hunk) were evaluated for Hunk mRNA expression levels by Quantitative-RealTime PCR to show the efficiency of knockdown. Hunk levels were normalized to Gapdh. Western blot analysis of HUNK showed reduced protein expression in Hunk shRNA cells compared to control cells. (B) Control and HUNK knockdown SMF cells were treated with either DMSO, growth factor deprivation (0% FBS, no insulin), or STU for 24 hours and analyzed for caspase-3 activity (C) SMF cells were treated with either DMSO or STU for 24 hours at the indicated concentration. Immunoblotting for PKC activity was performed using anti-pPKC substrate and pPKC antibodies. Total PKC was also evaluated. (D) SMF cells were treated with either DMSO or STU for 24 hours at the indicated concentration. Immunoblotting for p27 was performed using anti-p27 antibody. (E) SMF cells plated on coverslips were treated with either DMSO or 1 nM STU for 24 hours before fixation and subsequent confocal imaging. (F) Percent of cells containing nuclear p27 in SMF cells treated with DMSO or STU. {KD}

Immunogen image

Immunogen Image HUNK Polyclonal Antibody

产品信息

PA5-28765

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

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产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HUNK. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.65 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546241

产品详细信息

Recommended positive controls: A431, HeLa.

Predicted reactivity: Chimpanzee (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

HUNK serine/threonine kinase or hormonally up-regulated Neu-associated kinase is a member of the mitogen-activated protein kinase family which is essential for mammary tumor metastasis. HUNK deletion in mice did not alter viability, fertility, longevity, or propensity to develop tumors and it is also dispensable for murine development. HUNK has been shown to participate in vesicular transport. Additionally, functional studies in mice suggest that HUNK regulates endocytosis via interaction with rabaptin-5 protein and induces changes in mammary glands during pregnancy. HUNK expression has been documented in mouse brain, breast, embryo, and fetus. ESTs have been isolated from several human tissue libraries, including normal ear.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B19; Hormonally up-regulated neu tumor-associated kinase; hormonally upregulated neu tumor-associated kinase; hormonally upregulated Neu-associated kinase; serine/threonine protein kinase MAK-V; Serine/threonine-protein kinase MAK-V

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基因别名: HUNK; MAKV

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UniProt ID: (Human) P57058

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Entrez Gene ID: (Human) 30811

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功能

protein serine/threonine kinase activity ATP binding non-receptor serine/threonine protein kinase protein modifying enzyme

参与通路

protein phosphorylation signal transduction multicellular organism development intracellular signal transduction