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The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.
The antibody may be stored at 4 C. For prolonged storage prepare appropriate aliquots and store at or below -20 C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; Alpha-globin; ALPHA-GLOBIN GENE; beta globin chain; Beta-globin; delta globin; Haemoglobin; Hb; HbA1c; hemoglobin alpha 1 globin chain; Hemoglobin alpha chain; Hemoglobin beta chain; Hemoglobin subunit alpha; Hemoglobin subunit beta; hemoglobin, alpha 1; hemoglobin, beta
基因别名: beta-globin; CD113t-C; HBA-T3; HBA1; HBA2; HBB; HBH
UniProt ID: (Human) P69905, (Human) P68871
Entrez Gene ID: (Human) 3039, (Human) 3043