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Chromotek
Halo VHH Recombinant Alpaca Monoclonal Antibody (CTK0213)
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产品信息
OT-250
产品规格
种属反应
Tag
宿主/亚型
Alpaca
Expression System
E. coli
分类
Recombinant Monoclonal
类型
Antibody
克隆号
CTK0213
抗原
Halo protein from Branchiostoma lanceolatum
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
25mM TAPS, pH 8.5, with 5mM EDTA, 500mM NaCl
内含物
0.09% sodium azide
保存条件
4°C, do not freeze
运输条件
Wet ice
产品详细信息
Anti-Halo Nanobody for conjugation to beads, plates and surfaces via NHS-chemistry.
Affinity: Dissociation constant KD of 2 nM. Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
靶标信息
The HaloTag is a self-labeling protein derived from the haloalkane dehalogenase enzyme DhaA from Rhodococcus rhodochrous. It can be used as protein tag for tagging your protein of interest (POI). After transfection of your cell line/organism with a plasmid encoding for your Halo-tagged protein, the Halo-tagged protein is expressed. Through incubation of your cell line/organism with specific ligands (see below point 6), your Halo fusion protein turns into a fluorescent protein by binding a fluorescent dye or acquires biotin for immobilization or other streptavidin modifications. Once the HaloTag has formed a complex with a ligand, the catalytic center is not accessible for additional ligands. Alternatively, the Halo fusion protein can be detected or immunoprecipitated by antibodies or Nanobodies binding outside of its catalytic center. The HaloTag consists of 297 amino acids and has a molecular weight of 33 kDa. The HaloTag is derived from haloalkane dehalogenase enzyme DhaA. Its active site has been genetically modified. Chloroalkane linker substrates are irreversibly bound to the active site.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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