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GenScript
His Tag (C-term) Recombinant Rabbit Monoclonal Antibody (25B6E11), MonoRab™
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His Tag (C-term) Antibody (A01857-40) in WB
Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) Loading: Lane 1: MonoRab™ Anti-His antibody (4 µg) + His tagged fusion protein Lane 2: Rabbit IgG (4 µg) + His tagged fusion protein Lane 3: His tagged fusion protein (Input) Primary Antibody: THE™ His tag antibody, mAb, Mouse (Genscript,A01857) 0.2 µg/mL Secondary Antibody: Goat anti-rabbit IgG (H&L) [IRDye 800 ] (Licor,926-32211) 0.125 µg/mL.
产品信息
A01857-40
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
Expression System
Mammalian cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
25B6E11
抗原
6x His synthetic peptide coupled to KLH
偶联物
形式
Lyophilized
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high affinity and specific monoclonal rabbit antibodies. GenScript MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
靶标信息
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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