Invitrogen

Histone H2A.X Polyclonal Antibody

Histone H2A.X Antibody in Immunocytochemistry (ICC/IF)

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Histone H2A.X Antibody (PA5-28778) in ICC/IF

Histone H2A. X Polyclonal Antibody detects Histone H2A. X protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A. X stained by Histone H2A. X Polyclonal Antibody (Product # PA5-28778) diluted at 1:2,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Histone H2A.X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Histone H2A.X Antibody in Western Blot (WB)

Histone H2A.X Antibody in Immunoprecipitation (IP)

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产品信息

PA5-28778

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:20,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.41 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546254

产品详细信息

Recommended positive controls: 293T, HepG2, HCT116, HCT116 (30uM cisplatin for 24hr), Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, mouse brain, rat brain, PC-12, Rat2, 4T1.

Predicted reactivity: Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transglutaminase type 2-dependent crosslinking of IRF3 in dying melanoma cells.

Cell death discovery

Occhigrossi L,D'Eletto M,Vecchio A,Piacentini M,Rossin F

PA5-28778 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that TG2 negatively regulates the IRF3 pathway in human melanoma cells suggesting a so far unknown TG2-dependent mechanism by which cancer cells reduce the IFNI production after DNA damage to limit the immune system response.

Mon Dec 26 00:00:00 UTC 2022

The gut microbiota metabolite urolithin A inhibits NF-κB activation in LPS stimulated BMDMs.

Scientific reports

Abdelazeem KNM,Kalo MZ,Beer-Hammer S,Lang F

PA5-28778 was used in Immunocytochemistry-immunoflourescence to investigated the effect of urolithin A on the suppression of pro-inflammatory molecules and NF-κB activation by targeting TLR4 signalling pathway in BMDMs.

Mon Mar 29 00:00:00 UTC 2021

The gut microbiota metabolite urolithin A inhibits NF-κB activation in LPS stimulated BMDMs.

Scientific reports

Abdelazeem KNM,Kalo MZ,Beer-Hammer S,Lang F

Mon Mar 29 00:00:00 UTC 2021

Rapamycin-PLGA microparticles prevent senescence, sustain cartilage matrix production under stress and exhibit prolonged retention in mouse joints.

Biomaterials science

Dhanabalan KM,Gupta VK,Agarwal R

PA5-28778 was used in Immunocytochemistry to show that such particulate systems are promising candidates for intra-articular delivery of rapamycin for the treatment of osteoarthritis.