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Invitrogen
Human Glucocorticoid Receptor beta (aa 728-742) Synthetic Peptide
产品信息
PEP-222
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Not Applicable |
分类 |
Synthetic |
类型 |
Peptide |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
0.5 mg/mL |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
产品详细信息
PEP-222 is a 16 amino acid synthetic peptide corresponding to amino acid residues 728-742 from human GR beta. The sequence of this peptide is (amino to carboxy terminus): N (728) V M W L K P E S T S H T L I (742) C
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product, catalog # PA3-514. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.
Reconstitute with 0.1 mL of distilled water.
靶标信息
Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR beta might function as a dominant negative inhibitor of GR alpha activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GR; GR Beta; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
基因别名: GRL; NR3C1
