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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
Human Midkine recombinant protein contains 123 amino acids |
分子量 |
13.4 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 = 0.1 - 10 ng/mL; determined by the dose-dependent chemotaxis of human neutrophils. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
-20°C |
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile, distilled water to a concentration of 0.1-1 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Amphiregulin-associated protein; ARAP; FLJ27379; Midgestation and kidney protein; Midkine; MK; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; retinoic acid inducible factor
基因别名: ARAP; MDK; MK; MK1; NEGF2
UniProt ID: (Human) P21741
Entrez Gene ID: (Human) 4192
分子生物学功能:
growth factor
