Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属反应 |
Human |
宿主/亚型 |
Not Applicable |
分类 |
Synthetic |
类型 |
Peptide |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
0.5 mg/mL |
纯化类型 |
purified |
内含物 |
no preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
PEP-027 is a 16 amino acid synthetic peptide whose sequence is derived from human PPARg2 and is absent in PPARg1. A COOH-terminal cysteine residue was added for conjugation to KLH. The sequence of this peptide is (amino to carboxy terminus): M (1) G E T L G D S P I D P E S D S (16) C
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and PPARg2, catalog # PA1-824. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.
Reconstitute with 0.1 mL of distilled water.
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR-gamma
基因别名: CIMT1; GLM1; NR1C3; PPARG; PPARG1; PPARG2; PPARgamma
UniProt ID: (Human) P37231
Entrez Gene ID: (Human) 5468
分子生物学功能:
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator