Invitrogen

IBSP Polyclonal Antibody

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IBSP Antibody in Immunohistochemistry (IHC) — Fig. 1 Bone-metastatic ER + breast cancer cells secrete miR-19a and IBSP. a EVs were prepared from the conditioned medium of bone-metastatic cell line MCF7BoM2 and the parental cell line MCF7, followed by extraction of RNA. They were then subjected to miRNA profiling by GeneAtlas miRNA Array. Using the criteria of FDR < 0.05 and Fold change > 1.3, 156 miRNAs were selected for further study. These miRNAs were then examined whether they are secreted in the blood of ER + patients using the cohort data set (GSE41922) of the circulating microRNA profiles between 23 ER + breast cancer (BC) patients and 22 healthy donors (Normal). Three miRNAs (miR-19a. miR-133b, miR-576-5p), were found to be significantly upregulated (FDR < 0.05 and logFC > 0.5) in the blood of breast cancer patients. BoM, bone metastatic. b Kaplan-Meier analysis of miR-19a was performed between breast cancer patients with low miR-19a and high miR-19a expression using GSE22220. c RNA from bone-met(metastatic) lesions ( n = 6), primary tumors ( n = 6), lung and brain lesions ( n = 6) of ER + breast cancer patients, and normal bones ( n = 4) was extracted and miR-19a expression was examined by Taqman-PCR. A two-sided student's t -test was performed (Primary vs Bone-met, p = 0.0047; Other-met vs Bone-met, p = 0.0249; Normal Bone vs Bone-met, p = 0.0059). Data are presented as mean values +- SEM. d The expression of miR-19a in parental cell lines (MCF7, T47D, and MDA-MB-231) and bone-tropic cell lines (MCF7BoM2, T47DBo

产品信息

PA5-50633

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:200-1:1,000	-
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:25-1:100	-

产品规格
种属反应	Human
已发表种属	Not Applicable
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide corresponding to a region derived from internal residues of human integrin-binding sialoprotein
偶联物	Unconjugated
形式	Liquid
浓度	1.4 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2636086

产品详细信息

The antibody detects endogenous levels of total IBSP protein.

靶标信息

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer.

Nature communications

Wu K,Feng J,Lyu F,Xing F,Sharma S,Liu Y,Wu SY,Zhao D,Tyagi A,Deshpande RP,Pei X,Ruiz MG,Takahashi H,Tsuzuki S,Kimura T,Mo YY,Shiozawa Y,Singh R,Watabe K

Published figure using IBSP polyclonal antibody (Product # PA5-50633) in Immunohistochemistry

Tue Aug 31 00:00:00 EDT 2021

生物信息学

蛋白别名: Bone sialoprotein 2; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein

基因别名: BNSP; BSP; BSP-II; IBSP; SP-II

UniProt ID: (Human) P21815

Entrez Gene ID: (Human) 3381

分子生物学功能: extracellular matrix protein extracellular matrix structural protein

功能

osteoblast differentiation cell adhesion extracellular matrix organization bone mineralization cellular response to growth factor stimulus

参与通路

molecular_function

IBSP Polyclonal Antibody

产品信息

PA5-50633

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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