Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:1,000 | - |
免疫沉淀 (IP) |
1:50 | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Rabbit / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Synthetic peptide corresponding to residues surrounding Asp224 of human DFF45 |
偶联物 |
Unconjugated |
形式 |
Liquid |
纯化类型 |
Antigen affinity chromatography |
保存液 |
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
内含物 |
no preservative |
保存条件 |
-20°C |
RRID |
AB_10985915 |
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with related proteins.
ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: DFF; DFF-45; DFF45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD; RP23-121D17.3
基因别名: DFF-45; DFF1; DFF45; DFFA; H13; ICAD
UniProt ID: (Human) O00273
Entrez Gene ID: (Human) 1676
