Invitrogen

ID2 Monoclonal Antibody (ILCID2), eFluor™ 450, eBioscience™

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ID2 Antibody (48-9475-82) in Flow

C57BL/6 mouse splenocytes stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with either 1.0 µg of Mouse IgG1 kappa Isotype Control, eFluor 450 (Product # 48-4714-82) (left) or 1.0 µg of ID2 Monoclonal Antibody, eFluor 450 (right). Cells were co-stained and gated based on the expression of both NK1.1 Monoclonal Antibody, APC (Product # 17-5941-82) and CD49b Monoclonal Antibody, APC (Product # 17-5971-82) (purple histogram); CD4 Monoclonal Antibody, FITC (Product # 11-0042-... View More

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ID2 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, ID2 clone ILCID2 stains CD56+ lymphocytes but not CD56- lymphocytes. Details: Human PBMC were surface stained with CD56 (clone TULY56) and CD3 (clone UCHT1), followed by intracellular staining with ID2 (clone ILCID2) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD56+ (purple histogram), CD3+CD56- (orange histogram), and CD3-CD56- (blue histogram) gates were used for analysis. {RE}

产品信息

48-9475-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1.0 µg/test

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ILCID2

抗原

Purified recombinant fragment of human ID2, corresponding to amino acids 1-134 expressed in E. coli

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偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2735053

产品详细信息

Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol.

Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor™ 450 is an alternative for Pacific Blue™. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

靶标信息

The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Progesterone attenuates Th17-cell pathogenicity in autoimmune uveitis via Id2/Pim1 axis.

Journal of neuroinflammation

Liu X,Gu C,Lv J,Jiang Q,Ding W,Huang Z,Liu Y,Su Y,Zhang C,Xu Z,Wang X,Su W

Published figure using ID2 monoclonal antibody (Product # 48-9475-82) in Flow Cytometry

Wed Jun 21 00:00:00 UTC 2023

OX40 agonism enhances PD-L1 checkpoint blockade by shifting the cytotoxic T cell differentiation spectrum.

Cell reports. Medicine

van der Sluis TC,Beyrend G,van der Gracht ETI,Abdelaal T,Jochems SP,Belderbos RA,Wesselink TH,van Duikeren S,van Haften FJ,Redeker A,Ouboter LF,Beyranvand Nejad E,Camps M,Franken KLMC,Linssen MM,Hohenstein P,de Miranda NFCC,Mei H,Bins AD,Haanen JBAG,Aerts JG,Ossendorp F,Arens R

48-9475-82 was used in Flow Cytometry to interrogate whether the landscape of T cell states in the peripheral blood predict responses to combinatorial targeting of the OX40 costimulatory and PD-1 inhibitory pathways.

Tue Mar 21 00:00:00 UTC 2023

Insulin-like Growth Factor 1 Supports a Pulmonary Niche that Promotes Type 3 Innate Lymphoid Cell Development in Newborn Lungs.

Immunity

Oherle K,Acker E,Bonfield M,Wang T,Gray J,Lang I,Bridges J,Lewkowich I,Xu Y,Ahlfeld S,Zacharias W,Alenghat T,Deshmukh H

Published figure using ID2 monoclonal antibody (Product # 48-9475-82) in Flow Cytometry

Tue Feb 18 00:00:00 UTC 2020

生物信息学

蛋白别名: bHLHb26; cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID2; helix-loop-helix protein ID2; Inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; MGC26389; OTTHUMP00000140258; OTTHUMP00000200297

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基因别名: AI255428; BHLHB26; C78922; GIG8; Id-2; ID2; ID2A; ID2H; Idb2

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UniProt ID: (Human) Q02363, (Mouse) P41136

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Entrez Gene ID: (Human) 3398, (Mouse) 15902

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功能

protein binding ion channel binding protein dimerization activity DNA-binding transcription factor

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