Invitrogen

IFN beta Polyclonal Antibody

IFN beta Antibody in Immunocytochemistry (ICC/IF)

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IFN beta Antibody (PA5-20390) in ICC/IF

Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling IFN-beta with IFN beta Polyclonal Antibody (Product # PA5-20390) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

IFN beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFN beta Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

IFN beta Antibody in Immunohistochemistry (IHC)

IFN beta Antibody in Immunohistochemistry, ELISA (IHC, ELISA)

IFN beta Antibody in Western Blot, ELISA (WB, ELISA)

IFN beta Antibody in Flow Cytometry (Flow)

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产品信息

PA5-20390

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

2.5 µg/mL

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

20 µg/mL

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

20 µg/mL

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 17 amino acid peptide from near the center of human IFN-b.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11155641

产品详细信息

A suggested positive control is A-20 cell lysate.

PA5-20390 can be used with blocking peptide PEP-0507.

靶标信息

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Crosstalk between TBK1/IKKε and the type I interferon pathway contributes to tubulointerstitial inflammation and kidney tubular injury.

Frontiers in pharmacology

Córdoba-David G,García-Giménez J,Cardoso Castelo-Branco R,Carrasco S,Cannata P,Ortiz A,Ramos AM

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Immunohistochemistry

Fri Nov 18 00:00:00 UTC 2022

Crosstalk between TBK1/IKKε and the type I interferon pathway contributes to tubulointerstitial inflammation and kidney tubular injury.

Frontiers in pharmacology

Córdoba-David G,García-Giménez J,Cardoso Castelo-Branco R,Carrasco S,Cannata P,Ortiz A,Ramos AM

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Immunohistochemistry

Fri Nov 18 00:00:00 UTC 2022

1,25(OH)₂ D₃ blocks IFNβ production through regulating STING in epithelial layer of oral lichen planus.

Journal of cellular and molecular medicine

Ge X,Wang Y,Xie H,Li R,Zhang F,Zhao B,Du J

PA5-20390 was used in Western Blotting to find 1,25(OH)2 D3 regulates STING and IFNβ through a mechanism controlled by the hypoxia-inducible factor-1α (HIF-1α)-GATA1 axis.

Fri Jul 01 00:00:00 UTC 2022

dsRNA Induced IFNβ-MMP13 Axis Drives Corneal Wound Healing.

Investigative ophthalmology & visual science

Lan X,Zhang W,Zhu J,Huang H,Mo K,Guo H,Zhu L,Liu J,Li M,Wang L,Liu C,Ji J,Ouyang H

PA5-20390 was used in Immunohistochemistry, Western Blot, Immunhistochemistry (Paraffin) to identify MMP13 as a downstream effector of IFNβ where its expression promotes LSC proliferation and enhances corneal epithelial reconstruction in vivo.

Tue Feb 01 00:00:00 UTC 2022

In vitro and in vivo correlation of skin and cellular responses to nucleic acid delivery.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Bosnjak M,Znidar K,Sales Conniff A,Jesenko T,Markelc B,Semenova N,Tur J,Kohena K,Kranjc Brezar S,Heller L,Cemazar M

PA5-20390 was used in Immunohistochemistry-frozen immunofluorescence to investigate the immune response in skin cells, including keratinocytes, fibroblasts, and macrophages, following plasmid DNA electrotransfer, revealing upregulation of DNA sensors and cytokines.

Wed Jun 01 00:00:00 UTC 2022

Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy.

British journal of cancer

Broad RV,Jones SJ,Teske MC,Wastall LM,Hanby AM,Thorne JL,Hughes TA

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy.

British journal of cancer

Broad RV,Jones SJ,Teske MC,Wastall LM,Hanby AM,Thorne JL,Hughes TA

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Klotho deficiency intensifies hypoxia-induced expression of IFN-α/β through upregulation of RIG-I in kidneys.

PloS one

Urabe A,Doi S,Nakashima A,Ike T,Morii K,Sasaki K,Doi T,Arihiro K,Masaki T

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in ELISA

Mon Nov 29 00:00:00 UTC 2021

Klotho deficiency intensifies hypoxia-induced expression of IFN-α/β through upregulation of RIG-I in kidneys.

PloS one

Urabe A,Doi S,Nakashima A,Ike T,Morii K,Sasaki K,Doi T,Arihiro K,Masaki T

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in ELISA

Mon Nov 29 00:00:00 UTC 2021

IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer.

Cell reports

Ka NL,Lim GY,Hwang S,Kim SS,Lee MO

PA5-20390 was used in Immunohistochemistry to suggest that type-I IFNs and IFI16 could offer potential therapeutic strategies for TNBC.

Tue Dec 21 00:00:00 UTC 2021

Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity.

Science advances

Liang J,Wang H,Ding W,Huang J,Zhou X,Wang H,Dong X,Li G,Chen E,Zhou F,Fan H,Xia J,Shen B,Cai D,Lan P,Jiang H,Ling J,Cheng Z,Liu X,Sun J

PA5-20390 was used in Immunohistochemistry to offer a rational design of an effective immunotherapy combination regimen to convert uninflamed "cold" tumors into "hot" tumors, addressing the major challenges immunotherapies faced.

Sat Aug 01 00:00:00 UTC 2020

IFN-γ enhances cell-mediated cytotoxicity against keratinocytes via JAK2/STAT1 in lichen planus.

Science translational medicine

Shao S,Tsoi LC,Sarkar MK,Xing X,Xue K,Uppala R,Berthier CC,Zeng C,Patrick M,Billi AC,Fullmer J,Beamer MA,Perez-White B,Getsios S,Schuler A,Voorhees JJ,Choi S,Harms P,Kahlenberg JM,Gudjonsson JE

PA5-20390 was used in Immunohistochemistry to study the role and mechanisms of IFN-γ in Lichen planus pathogenesis and provides evidence for the therapeutic use of JAK inhibitors to limit cell-mediated cytotoxicity in patients with Lichen planus.

Wed Sep 25 00:00:00 UTC 2019

RIPK3 interacts with MAVS to regulate type I IFN-mediated immunity to Influenza A virus infection.

PLoS pathogens

Downey J,Pernet E,Coulombe F,Allard B,Meunier I,Jaworska J,Qureshi S,Vinh DC,Martin JG,Joubert P,Divangahi M

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Immunofluorescence

Sat Apr 01 00:00:00 UTC 2017

Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunum.

Journal of lipid research

Mukherjee P,Hough G,Chattopadhyay A,Navab M,Fogelman HR,Meriwether D,Williams K,Bensinger S,Moller T,Faull KF,Lusis AJ,Iruela-Arispe ML,Bostrom KI,Tontonoz P,Reddy ST,Fogelman AM

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in ELISA

Tue Aug 01 00:00:00 UTC 2017

Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunum.

Journal of lipid research

Mukherjee P,Hough G,Chattopadhyay A,Navab M,Fogelman HR,Meriwether D,Williams K,Bensinger S,Moller T,Faull KF,Lusis AJ,Iruela-Arispe ML,Bostrom KI,Tontonoz P,Reddy ST,Fogelman AM

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in ELISA

Tue Aug 01 00:00:00 UTC 2017

STING activation of tumor endothelial cells initiates spontaneous and therapeutic antitumor immunity.

Proceedings of the National Academy of Sciences of the United States of America

Demaria O,De Gassart A,Coso S,Gestermann N,Di Domizio J,Flatz L,Gaide O,Michielin O,Hwu P,Petrova TV,Martinon F,Modlin RL,Speiser DE,Gilliet M

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Flow Cytometry

Tue Dec 15 00:00:00 UTC 2015

STING activation of tumor endothelial cells initiates spontaneous and therapeutic antitumor immunity.

Proceedings of the National Academy of Sciences of the United States of America

Demaria O,De Gassart A,Coso S,Gestermann N,Di Domizio J,Flatz L,Gaide O,Michielin O,Hwu P,Petrova TV,Martinon F,Modlin RL,Speiser DE,Gilliet M

Published figure using IFN beta polyclonal antibody (Product # PA5-20390) in Flow Cytometry

Tue Dec 15 00:00:00 UTC 2015

生物信息学

蛋白别名: Fibroblast interferon; IFN b; IFN β; IFN-beta; IFNbeta; IFNβ; Interferon; Interferon b; Interferon beta; interferon, beta 1, fibroblast; interferon-beta; RP11-113D19.1

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基因别名: IFB; IFF; IFN-beta; IFNB; IFNB1

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UniProt ID: (Human) P01574, (Mouse) P01575

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Entrez Gene ID: (Human) 3456, (Mouse) 15977

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功能

cytokine activity type I interferon receptor binding chloramphenicol O-acetyltransferase activity cytokine receptor binding interferon superfamily

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