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产品信息
PBOIFNGBI
产品规格
种属反应
Bovine
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Bovine IFN gamma
偶联物
Biotin
形式
Lyophilized
浓度
0.25 mg/mL
纯化类型
purified
保存液
PBS
内含物
proprietary preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10979934
产品详细信息
Reconstitute with 1mL of deionized water for a final concentration of 0.25mg/mL.
PBOIFNGBI targets IFN gamma in ELISA applications and shows reactivity with Bovine samples.
The PBOIFNGBI immunogen is recombinant Bovine IFN gamma.
PBOIFNGBI detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.
The PBOIFNGBI polyclonal biotinylated bovine IFNg antibody has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody PBOIFNGI. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
靶标信息
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BoIFNG; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; interferon gamma type 2; Interferon y; Interferon γ; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
基因别名: IFNG
UniProt ID: (Bovine) P07353
Entrez Gene ID: (Bovine) 281237
microglial cell activation
macrophage activation involved in immune response
cell cycle arrest
positive regulation of autophagy
positive regulation of epithelial cell migration
regulation of protein ADP-ribosylation
macrophage differentiation
positive regulation of B cell proliferation
positive regulation of protein complex assembly
negative regulation of interleukin-17 production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
regulation of growth
positive regulation of protein import into nucleus
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of chemokine biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
astrocyte activation
negative regulation of smooth muscle cell proliferation
positive regulation of cytokine secretion
positive regulation of inflammatory response
positive regulation of neurogenesis
regulation of insulin secretion
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
positive regulation of killing of cells of other organism
interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of calcidiol 1-monooxygenase activity
positive regulation of protein serine/threonine kinase activity
positive regulation of protein deacetylation
extrinsic apoptotic signaling pathway
positive regulation of neuron death
positive regulation of beta-amyloid formation
negative regulation of tau-protein kinase activity
positive regulation of core promoter binding
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
