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Invitrogen
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
.jpg "IL-1 alpha Antibody (PA5-47011) in IHC (F)")
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
0.1 µg/mL | - |
免疫组化（冰冻） (IHC (F)) |
5-15 µg/mL | - |
中和实验 (Neu) |
0.002-0.004 µg/mL | - |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
宿主/亚型 |
Goat / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
E. coli-derived recombinant mouse IL-1 alpha/IL-1F1 Ser6-Ser161 |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
0.2 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS with 5% trehalose |
内含物 |
No Preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2609803 |
In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL-1 alpha and recombinant cotton rat IL-1 alpha is observed and less than 1% cross-reactivity with recombinant human IL-1 alpha is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; RP23-160G19.8
基因别名: Il-1a; Il1a
UniProt ID: (Mouse) P01582
Entrez Gene ID: (Mouse) 16175
分子生物学功能:
interleukin superfamily
