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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1 µg/mL | - |
免疫细胞化学 (ICC/IF) |
8-25 µg/mL | - |
酶联免疫吸附实验 (ELISA) |
0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture) | - |
中和实验 (Neu) |
0.05-0.15 µg/mL | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
宿主/亚型 |
Mouse / IgG2a |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
4414 |
抗原 |
E. coli-derived recombinant human IL-1 alpha/IL-1F1 Ser113-Ala271 |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
0.5 mg/mL |
纯化类型 |
Protein A/G |
保存液 |
PBS with 5% trehalose |
内含物 |
No Preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2609800 |
In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CTLA8; Hematopoietin-1; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 1, alpha; interleukin 17; Interleukin-1 alpha; Interleukin1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8
基因别名: IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552
分子生物学功能:
interleukin superfamily
