Invitrogen

IL-1 beta Monoclonal Antibody (B122), eBioscience™

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IL-1 beta Antibody (14-7012-81) in ELISA

Chonnam medical journal 2020 - FIG. 1 Differential activation between tissue-resident and TG-elicited peritoneal macrophages from young mice. Peritoneum-resident (Mph)- and TG-elicited peritoneum macrophages (TG-Mph) were isolated from young mice (Y, 8 weeks old). Total cell numbers (n=5/each group) were counted (A), and phagocytotic activity (n=5/each group) was quantified (B). The ratio for Mph was arbitrarily defined as 100%, and the ... View More

IL-1 beta Antibody in Neutralization (Neu)

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产品信息

14-7012-81

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1-4 µg/mL

查看14篇已发表文章 14篇已发表文章

中和实验 (Neu)

Assay-Dependent

查看8篇已发表文章 8篇已发表文章

功能检测 (Functional)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

B122

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_468396

产品详细信息

Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.

Applications Reported: The B122 antibody has been reported for use in cytokine neutralization, immunoblotting (WB), and ELISA. Please refer to Functional Grade purified B122 (Product # 16-7012) for functional assays.

Applications Tested: The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotinylated polyclonal anti-mouse interleukin-1 beta for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Chronic kidney disease promotes atrial fibrillation via inflammasome pathway activation.

The Journal of clinical investigation

Song J,Navarro-Garcia JA,Wu J,Saljic A,Abu-Taha I,Li L,Lahiri SK,Keefe JA,Aguilar-Sanchez Y,Moore OM,Yuan Y,Wang X,Kamler M,Mitch WE,Ruiz-Hurtado G,Hu Z,Thomas SS,Dobrev D,Wehrens XH,Li N

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in Neutralization

Mon Oct 02 00:00:00 UTC 2023

Origin and Function of Stress-Induced IL-6 in Murine Models.

Cell

Qing H,Desrouleaux R,Israni-Winger K,Mineur YS,Fogelman N,Zhang C,Rashed S,Palm NW,Sinha R,Picciotto MR,Perry RJ,Wang A

14-7012-81 was used in ELISA to study the cytokine interleukin-6 (IL-6) and its induction following acute stress in mouse models.

Thu Jul 23 00:00:00 UTC 2020

Acetate coordinates neutrophil and ILC3 responses against C. difficile through FFAR2.

The Journal of experimental medicine

Fachi JL,Sécca C,Rodrigues PB,Mato FCP,Di Luccia B,Felipe JS,Pral LP,Rungue M,Rocha VM,Sato FT,Sampaio U,Clerici MTPS,Rodrigues HG,Câmara NOS,Consonni SR,Vieira AT,Oliveira SC,Mackay CR,Layden BT,Bortoluci KR,Colonna M,Vinolo MAR

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in Neutralization

Mon Mar 02 00:00:00 UTC 2020

Differential Activation of Macrophages Based on Their Environment in Advanced Age.

Chonnam medical journal

Lee DY,Lim JS,Cho KA

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in ELISA

Wed Jan 01 00:00:00 UTC 2020

NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma.

Molecular neurodegeneration

Chen H,Deng Y,Gan X,Li Y,Huang W,Lu L,Wei L,Su L,Luo J,Zou B,Hong Y,Cao Y,Liu Y,Chi W

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in Neutralization

Wed Apr 15 00:00:00 UTC 2020

NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma.

Molecular neurodegeneration

Chen H,Deng Y,Gan X,Li Y,Huang W,Lu L,Wei L,Su L,Luo J,Zou B,Hong Y,Cao Y,Liu Y,Chi W

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in Neutralization

Wed Apr 15 00:00:00 UTC 2020

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in ELISA

Wed May 29 00:00:00 UTC 2019

A Type I Interferon and IL-10 Induced by Orientia tsutsugamushi Infection Suppresses Antigen-Specific T Cells and Their Memory Responses.

Frontiers in immunology

Min CK,Kim HI,Ha NY,Kim Y,Kwon EK,Yen NTH,Youn JI,Jeon YK,Inn KS,Choi MS,Cho NH

14-7012 was used in Neutralization experiments to show that the induction of the type I IFN/IL-10 axis by O. tsutsugamushi infection might play a significant role in the suppression of T cell responses and contribute to the short longevity of cell-mediated immunity, often observed in scrub typhus patients.

Mon Sep 30 00:00:00 UTC 2019

Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission.

Cell

Rao S,Schieber AMP,O'Connor CP,Leblanc M,Michel D,Ayres JS

14-7012 was used in an ELISA assay to study the mechanism by which Salmonella Typhimurium inhibits anorexia.

Thu Jan 26 00:00:00 UTC 2017

Intestinal fungi contribute to development of alcoholic liver disease.

The Journal of clinical investigation

Yang AM,Inamine T,Hochrath K,Chen P,Wang L,Llorente C,Bluemel S,Hartmann P,Xu J,Koyama Y,Kisseleva T,Torralba MG,Moncera K,Beeri K,Chen CS,Freese K,Hellerbrand C,Lee SM,Hoffman HM,Mehal WZ,Garcia-Tsao G,Mutlu EA,Keshavarzian A,Brown GD,Ho SB,Bataller R,Stärkel P,Fouts DE,Schnabl B

14-7012 was used in an ELISA assay to investigate the contribution of intestinal fungi, or mycobiota, to alcoholic liver disease.

Fri Jun 30 00:00:00 UTC 2017

Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion.

Frontiers in immunology

Shi G,Abbott KN,Wu W,Salter RD,Keyel PA

14-7012 was used in an ELISA assay to investigate the possibility that immune activation, specifically inflammasome activation, is regulated by Dnase1L3.

Fri Aug 04 00:00:00 UTC 2023

Orally administered sodium 4-phenylbutyrate suppresses the development of dextran sulfate sodium-induced colitis in mice.

Experimental and therapeutic medicine

Ono K,Nimura S,Hideshima Y,Nabeshima K,Nakashima M

14-7012 was used in an ELISA assay to investigate the effects of orally administered sodium 4-phenylbutyrate in mice.

Fri Dec 01 00:00:00 UTC 2017

Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology.

PloS one

Selby MJ,Engelhardt JJ,Johnston RJ,Lu LS,Han M,Thudium K,Yao D,Quigley M,Valle J,Wang C,Chen B,Cardarelli PM,Blanset D,Korman AJ

14-7012 was used in Flow cytometry/Cell sorting to exhibit a preclinical strategy for the identification and development of highly effective antitumour combination immunotherapies.

Fri Aug 25 00:00:00 UTC 2017

Cholera Toxin Promotes Th17 Cell Differentiation by Modulating Expression of Polarizing Cytokines and the Antigen-Presenting Potential of Dendritic Cells.

PloS one

Kang JO,Lee JB,Chang J

14-7012 was used in Neutralization experiments to determine how cholera toxin skews the differentiation of CD4 T cells to form IL-17-producing Th17 cells.

Tue Aug 08 00:00:00 UTC 2017

Cyclo(Phe-Pro) produced by the human pathogen Vibrio vulnificus inhibits host innate immune responses through the NF-κB pathway.

Infection and immunity

Kim K,Kim NJ,Kim SY,Kim IH,Kim KS,Lee GR

14-7012 was used in an ELISA assay to investigate the effects of a secondary metabolite produced by the lethal human bacterial pathogen Vibrio vulnificus on the mammalian innate immune system.

Sun Mar 01 00:00:00 UTC 2015

SENP1-mediated NEMO deSUMOylation in adipocytes limits inflammatory responses and type-1 diabetes progression.

Nature communications

Shao L,Zhou HJ,Zhang H,Qin L,Hwa J,Yun Z,Ji W,Min W

Published figure using IL-1 beta monoclonal antibody (Product # 14-7012-81) in ELISA

Tue Nov 24 00:00:00 UTC 2015

NLRC4 expression in intestinal epithelial cells mediates protection against an enteric pathogen.

Mucosal immunology

Nordlander S,Pott J,Maloy KJ

14-7012 was used in an ELISA assay to study how early NLRC4 sensing in intestinal epithelial cells regulates colonization by an extracellular bacterial pathogen and limits subsequent intestinal damage.

Tue Jul 01 00:00:00 UTC 2014

Nlrp3 activation in the intestinal epithelium protects against a mucosal pathogen.

Mucosal immunology

Song-Zhao GX,Srinivasan N,Pott J,Baban D,Frankel G,Maloy KJ

14-7012 was used in an ELISA assay to investigate how protection from disease is mediated through Nlrp3 activation in nonhematopoietic cells and is initiated very early after infection.

Tue Jul 01 00:00:00 UTC 2014

The alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles.

Particle and fibre toxicology

Rabolli V,Badissi AA,Devosse R,Uwambayinema F,Yakoub Y,Palmai-Pallag M,Lebrun A,De Gussem V,Couillin I,Ryffel B,Marbaix E,Lison D,Huaux F

14-7012 was used in Functional assays to assess the implication of alarmins (IL-1α, IL-33 and HMGB1) in the lung response to silica particles.

Sat Dec 13 00:00:00 UTC 2014

Type I interferon induces necroptosis in macrophages during infection with Salmonella enterica serovar Typhimurium.

Nature immunology

Robinson N,McComb S,Mulligan R,Dudani R,Krishnan L,Sad S

14-7012 was used in Neutralization experiments to propose that S. Typhimurium induces the production of type I interferon, which drives necroptosis of macrophages and allows them to evade the immune response.

Mon Oct 01 00:00:00 UTC 2012

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

14-7012 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation.

Fri Jul 01 00:00:00 UTC 2011

Interleukin 1 receptor signaling regulates DUBA expression and facilitates Toll-like receptor 9-driven antiinflammatory cytokine production.

The Journal of experimental medicine

González-Navajas JM,Law J,Nguyen KP,Bhargava M,Corr MP,Varki N,Eckmann L,Hoffman HM,Lee J,Raz E

14-7012 was used in Neutralization experiments to investigate the role of IL-1R in antiinflammatory cytokine production.

Mon Dec 20 00:00:00 UTC 2010

Interleukin 1 receptor signaling regulates DUBA expression and facilitates Toll-like receptor 9-driven antiinflammatory cytokine production.

The Journal of experimental medicine

González-Navajas JM,Law J,Nguyen KP,Bhargava M,Corr MP,Varki N,Eckmann L,Hoffman HM,Lee J,Raz E

14-7012 was used in Neutralization experiments to investigate the role of IL-1R in antiinflammatory cytokine production.

Mon Dec 20 00:00:00 UTC 2010

Tim3 binding to galectin-9 stimulates antimicrobial immunity.

The Journal of experimental medicine

Jayaraman P,Sada-Ovalle I,Beladi S,Anderson AC,Dardalhon V,Hotta C,Kuchroo VK,Behar SM

14-7012 was used in an ELISA assay to explore the role of the Th1 cell surface molecule Tim3 in the regulation of antimicrobial immunity.

Mon Oct 25 00:00:00 UTC 2010

Inflammation recapitulates the ontogeny of lymphoid stromal cells.

Journal of immunology (Baltimore, Md. : 1950)

Peduto L,Dulauroy S,Lochner M,Späth GF,Morales MA,Cumano A,Eberl G

14-7012 was used in Functional assays to show that LS cells are both programmed during ontogeny and reinduced during inflammation.

Fri May 01 00:00:00 UTC 2009

Transfer of tolerance to collagen type V suppresses T-helper-cell-17 lymphocyte-mediated acute lung transplant rejection.

Transplantation

Braun RK,Molitor-Dart M,Wigfield C,Xiang Z,Fain SB,Jankowska-Gan E,Seroogy CM,Burlingham WJ,Wilkes DS,Brand DD,Torrealba J,Love RB

14-7012 was used in Neutralization experiments to test whether Tregs from tolerant rats could suppress Th17-mediated rejection in the syngeneic model of lung transplantation.

Sun Dec 27 00:00:00 UTC 2009

生物信息学

蛋白别名: Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

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基因别名: Il-1b; IL-1beta; Il1b

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UniProt ID: (Mouse) P10749

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Entrez Gene ID: (Mouse) 16176, (Rat) 24494

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功能

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily

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