Invitrogen

IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), APC, eBioscience™

IL-1 beta (Pro-form) Antibody in Flow Cytometry (Flow)

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IL-1 beta (Pro-form) Antibody (17-7114-80) in Flow

Intracellular staining of BALB/c thioglycolate-elicited peritoneal macrophages unstimulated (left) or stimulated 6 hours with LPS (right) with Anti-Mouse CD14 PE (Product # 12-0141-82) and 0.03 µg of Anti-Mouse IL-1 beta Pro-form APC (right). Total viable cells were used for analysis.

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产品信息

17-7114-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看39篇已发表文章 39篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

NJTEN3

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10670739

产品详细信息

Description: This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. IL-1 beta signals via the IL-1RI, which is shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value. The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form.

Applications Reported: This NJTEN3 antibody has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: This NJTEN3 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions.IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Bi-directional neuro-immune dysfunction after chronic experimental brain injury.

Journal of neuroinflammation

Ritzel RM,Li Y,Jiao Y,Doran SJ,Khan N,Henry RJ,Brunner K,Loane DJ,Faden AI,Szeto GL,Wu J

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Fri Apr 05 00:00:00 UTC 2024

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Thu Jun 29 00:00:00 UTC 2023

Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes.

Autophagy

Hegdekar N,Sarkar C,Bustos S,Ritzel RM,Hanscom M,Ravishankar P,Philkana D,Wu J,Loane DJ,Lipinski MM

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

Low-dose adropin stimulates inflammasome activation of macrophage via mitochondrial ROS involved in colorectal cancer progression.

BMC cancer

Jia L,Liao L,Jiang Y,Hu X,Lu G,Xiao W,Gong W,Jia X

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Mon Oct 30 00:00:00 UTC 2023

Brain innate immune response via miRNA-TLR7 sensing in polymicrobial sepsis.

Brain, behavior, and immunity

Zou L,He J,Gu L,Shahror RA,Li Y,Cao T,Wang S,Zhu J,Huang H,Chen F,Fan X,Wu J,Chao W

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Tue Feb 01 00:00:00 UTC 2022

Stimulation of a subset of natural killer T cells by CD103⁺ DC is required for GM-CSF and protection from pneumococcal infection.

Cell reports

Murray MP,Crosby CM,Marcovecchio P,Hartmann N,Chandra S,Zhao M,Khurana A,Zahner SP,Clausen BE,Coleman FT,Mizgerd JP,Mikulski Z,Kronenberg M

17-7114-80 was used in Flow cytometry/Cell sorting to demonstrate how particular cellular interactions and responses of functional subsets of innate-like T cells contribute to protection from pathogenic lung infection.

Tue Jan 11 00:00:00 UTC 2022

Lysosomal acid lipase, CSF1R, and PD-L1 determine functions of CD11c+ myeloid-derived suppressor cells.

JCI insight

Zhao T,Liu S,Ding X,Johnson EM,Hanna NH,Singh K,Sen CK,Wan J,Du H,Yan C

17-7114-80 was used in Flow cytometry/Cell sorting to investigate if CD11c+ cells switched their functions to immune suppression and tumor growth stimulation through CSF1R/PD-L1 upregulation and metabolic reprogramming.

Thu Sep 08 00:00:00 UTC 2022

Neuroinflammation in neuronopathic Gaucher disease: Role of microglia and NK cells, biomarkers, and response to substrate reduction therapy.

eLife

Boddupalli CS,Nair S,Belinsky G,Gans J,Teeple E,Nguyen TH,Mehta S,Guo L,Kramer ML,Ruan J,Wang H,Davison M,Kumar D,Vidyadhara DJ,Zhang B,Klinger K,Mistry PK

17-7114-80 was used in Flow cytometry/Cell sorting to advance nGD disease biology whilst identifying compelling biomarkers of nGD to improve patient management, enrich clinical trials, and illuminate therapeutic targets.

Tue Aug 16 00:00:00 UTC 2022

Nitric oxide controls proliferation of Leishmania major by inhibiting the recruitment of permissive host cells.

Immunity

Formaglio P,Alabdullah M,Siokis A,Handschuh J,Sauerland I,Fu Y,Krone A,Gintschel P,Stettin J,Heyde S,Mohr J,Philipsen L,Schröder A,Robert PA,Zhao G,Khailaie S,Dudeck A,Bertrand J,Späth GF,Kahlfuß S,Bousso P,Schraven B,Huehn J,Binder S,Meyer-Hermann M,Müller AJ

17-7114-80 was used in Flow cytometry/Cell sorting to conclude that NO fulfills two distinct functions for L. major containment: permitting direct killing and restricting the supply of proliferation-permissive host cells.

Tue Dec 14 00:00:00 UTC 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Fri Mar 04 00:00:00 UTC 2022

NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Frontiers in immunology

Guo X,Xu X,Li T,Yu Q,Wang J,Chen Y,Ding S,Zhu L,Zou G,Zhang X

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Tue Jan 18 00:00:00 UTC 2022

MicroRNA-326 attenuates immune escape and prevents metastasis in lung adenocarcinoma by targeting PD-L1 and B7-H3.

Cell death discovery

Shao L,He Q,Wang J,He F,Lin S,Wu L,Gao Y,Ma W,Dong J,Yang X,Li F

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Tue Jun 15 00:00:00 UTC 2021

Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation.

Nature communications

Jones N,Blagih J,Zani F,Rees A,Hill DG,Jenkins BJ,Bull CJ,Moreira D,Bantan AIM,Cronin JG,Avancini D,Jones GW,Finlay DK,Vousden KH,Vincent EE,Thornton CA

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Mon Feb 22 00:00:00 UTC 2021

Interleukin-1β Suppresses Gastrin via Primary Cilia and Induces Antral Hyperplasia.

Cellular and molecular gastroenterology and hepatology

Ding L,Sontz EA,Saqui-Salces M,Merchant JL

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Tue Mar 08 00:00:00 UTC 2022

β-Glucan-stimulated neutrophil secretion of IL-1α is independent of GSDMD and mediated through extracellular vesicles.

Cell reports

Ratitong B,Marshall M,Pearlman E

17-7114-80 was used in Flow Cytometry to identify neutrophils as a source of IL-1α and demonstrate a role for EVs, specifically exosomes, in neutrophil secretion of bioactive IL-1α.

Tue May 18 00:00:00 UTC 2021

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Mon Jun 28 00:00:00 UTC 2021

NLRC4 inhibits NLRP3 inflammasome and abrogates effective antifungal CD8⁺ T cell responses.

iScience

Souza COS,Ketelut-Carneiro N,Milanezi CM,Faccioli LH,Gardinassi LG,Silva JS

17-7114-80 was used in Flow Cytometry to investigate if NLRC4 promotes disease by regulating the production of inflammatory cytokines and cellular responses that depend on the NLRP3 inflammasome activity.

Fri Jun 25 00:00:00 UTC 2021

GM-CSF production by non-classical monocytes controls antagonistic LPS-driven functions in allergic inflammation.

Cell reports

Kaur K,Bachus H,Lewis C,Papillion AM,Rosenberg AF,Ballesteros-Tato A,León B

17-7114-80 was used in Flow cytometry/Cell sorting to show here that lipopolysaccharide activity switches from pro-pathogenic to protective depending on the production of granulocyte-macrophage colony-stimulating factor by non-classical monocytes.

Tue Dec 28 00:00:00 UTC 2021

Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells.

Cell metabolism

Rosser EC,Piper CJM,Matei DE,Blair PA,Rendeiro AF,Orford M,Alber DG,Krausgruber T,Catalan D,Klein N,Manson JJ,Drozdov I,Bock C,Wedderburn LR,Eaton S,Mauri C

17-7114 was used in Flow cytometry/Cell sorting to investigate butyrate supplementation as a potential therapy for the amelioration of systemic autoimmune disorders.

Tue Apr 07 00:00:00 UTC 2020

Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation.

Cell host & microbe

Liu X,Boyer MA,Holmgren AM,Shin S

17-7114-80 was used in Flow Cytometry to reveal that alveolar macrophages engage alveolar epithelial signals to metabolically reprogram monocytes for antibacterial inflammation.

Wed Nov 11 00:00:00 UTC 2020

T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity.

Nature immunology

Jain A,Irizarry-Caro RA,McDaniel MM,Chawla AS,Carroll KR,Overcast GR,Philip NH,Oberst A,Chervonsky AV,Katz JD,Pasare C

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2020

T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity.

Nature immunology

Jain A,Irizarry-Caro RA,McDaniel MM,Chawla AS,Carroll KR,Overcast GR,Philip NH,Oberst A,Chervonsky AV,Katz JD,Pasare C

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2020

Hippocampal interleukin-33 mediates neuroinflammation-induced cognitive impairments.

Journal of neuroinflammation

Reverchon F,de Concini V,Larrigaldie V,Benmerzoug S,Briault S,Togbé D,Ryffel B,Quesniaux VFJ,Menuet A

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Fri Sep 11 00:00:00 UTC 2020

Triacylglycerol synthesis enhances macrophage inflammatory function.

Nature communications

Castoldi A,Monteiro LB,van Teijlingen Bakker N,Sanin DE,Rana N,Corrado M,Cameron AM,Hässler F,Matsushita M,Caputa G,Klein Geltink RI,Büscher J,Edwards-Hicks J,Pearce EL,Pearce EJ

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Fri Aug 14 00:00:00 UTC 2020

Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance.

Cell reports

DeSouza-Vieira T,Iniguez E,Serafim TD,de Castro W,Karmakar S,Disotuar MM,Cecilio P,Lacsina JR,Meneses C,Nagata BM,Cardoso S,Sonenshine DE,Moore IN,Borges VM,Dey R,Soares MP,Nakhasi HL,Oliveira F,Valenzuela JG,Kamhawi S

17-7114 was used in Flow cytometry/Cell sorting to demonstrate that HO-1 induction through erythrophagocytosis is a universal mechanism that regulates skin inflammation following blood feeding by arthropods, thus promoting early-stage disease tolerance to vector-borne pathogens.

Tue Oct 27 00:00:00 UTC 2020

GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation.

Cell reports

Castro-Dopico T,Fleming A,Dennison TW,Ferdinand JR,Harcourt K,Stewart BJ,Cader Z,Tuong ZK,Jing C,Lok LSC,Mathews RJ,Portet A,Kaser A,Clare S,Clatworthy MR

17-7114 was used in Flow cytometry/Cell sorting to identify granulocyte-macrophage colony stimulating factor (GM-CSF) as a critical regulator of intestinal macrophage activation in patients with IBD and mice with dextran sodium sulfate (DSS)-induced colitis.

Tue Jul 07 00:00:00 UTC 2020

Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis.

Immunity

Castro-Dopico T,Dennison TW,Ferdinand JR,Mathews RJ,Fleming A,Clift D,Stewart BJ,Jing C,Strongili K,Labzin LI,Monk EJM,Saeb-Parsy K,Bryant CE,Clare S,Parkes M,Clatworthy MR

17-7114-80 was used in Flow cytometry/Cell sorting to understand the mechanisms whereby changes in FcγRIIA affinity would affect inflammation in an IgA-dominated organ.

Tue Apr 16 00:00:00 UTC 2019

Toxin-Triggered Interleukin-1 Receptor Signaling Enables Early-Life Discrimination of Pathogenic versus Commensal Skin Bacteria.

Cell host & microbe

Leech JM,Dhariwala MO,Lowe MM,Chu K,Merana GR,Cornuot C,Weckel A,Ma JM,Leitner EG,Gonzalez JR,Vasquez KS,Diep BA,Scharschmidt TC

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Wed Dec 11 00:00:00 UTC 2019

Reprogramming the adjuvant properties of aluminum oxyhydroxide with nanoparticle technology.

NPJ vaccines

Orr MT,Khandhar AP,Seydoux E,Liang H,Gage E,Mikasa T,Beebe EL,Rintala ND,Persson KH,Ahniyaz A,Carter D,Reed SG,Fox CB

17-7114 was used in Flow cytometry/Cell sorting to determine whether the particle size and aggregated state of aluminum oxyhydroxide affects its adjuvant activity.

Thu Oct 01 00:00:00 UTC 2020

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Thu Sep 06 00:00:00 UTC 2018

Notch Regulates Macrophage-Mediated Inflammation in Diabetic Wound Healing.

Frontiers in immunology

Kimball AS,Joshi AD,Boniakowski AE,Schaller M,Chung J,Allen R,Bermick J,Carson WF,Henke PK,Maillard I,Kunkel SL,Gallagher KA

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota that Promotes Skin Inflammation.

Cell host & microbe

Gimblet C,Meisel JS,Loesche MA,Cole SD,Horwinski J,Novais FO,Misic AM,Bradley CW,Beiting DP,Rankin SC,Carvalho LP,Carvalho EM,Scott P,Grice EA

17-7114-80 was used in Flow Cytometry to investigate the role of skin microbiota in cutaneous leishmaniasis and found that human patients infected with Leishmania braziliensis develop dysbiotic skin microbiota, characterized by increases in the abundance of Staphylococcus and/or Streptococcus.

Wed Jul 12 00:00:00 UTC 2017

Dendritic cells, macrophages, NK and CD8⁺ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B.

Virology journal

Lucinda N,Figueiredo MM,Pessoa NL,Santos BS,Lima GK,Freitas AM,Machado AM,Kroon EG,Antonelli LR,Campos MA

17-7114 was used in Flow cytometry/Cell sorting to provide strong evidence that the responses mediated by DCs, Mo/M?, NK and CD8+ T lymphocytes through IL-1β, iNOS and granzyme B production, respectively, together with the production of type I IFN early in the infection, are crucial to host defense against HSV-1.

Tue Feb 21 00:00:00 UTC 2017

The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation.

Nature

Ravindran R,Loebbermann J,Nakaya HI,Khan N,Ma H,Gama L,Machiah DK,Lawson B,Hakimpour P,Wang YC,Li S,Sharma P,Kaufman RJ,Martinez J,Pulendran B

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Thu Mar 24 00:00:00 UTC 2016

Blockade of MK2 is protective in inflammation-associated colorectal cancer development.

International journal of cancer

Ray AL,Castillo EF,Morris KT,Nofchissey RA,Weston LL,Samedi VG,Hanson JA,Gaestel M,Pinchuk IV,Beswick EJ

17-7114 was used in Flow cytometry/Cell sorting to investigate the role of MAPK-activated protein kinase 2 in development of colitis-associated colon cancer.

Mon Feb 01 00:00:00 UTC 2016

Neutrophils Oppose Uterine Epithelial Carcinogenesis via Debridement of Hypoxic Tumor Cells.

Cancer cell

Blaisdell A,Crequer A,Columbus D,Daikoku T,Mittal K,Dey SK,Erlebacher A

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 17-7114-80) in Flow Cytometry

Mon Dec 14 00:00:00 UTC 2015

Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding.

PloS one

Jovicic N,Jeftic I,Jovanovic I,Radosavljevic G,Arsenijevic N,Lukic ML,Pejnovic N

17-7114 was used in Flow cytometry/Cell sorting to investigate the onset of obesity-associated metabolic diseases in two mouse strains with different immune phenotypes.