Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™

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IL-10 Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Human CD4 FITC (Product # 11-0049-42) and intracellularly stained with Anti-Human IL-10 PE.

产品信息

12-7108-82

应用	建议稀释比	已发表文章
流式细胞分析 (Flow)	0.25 µg/test	查看13篇已发表文章 13篇已发表文章
酶联免疫吸附实验 (ELISA)	-	查看1篇已发表文章 1篇已发表文章
功能检测 (FN)	-	查看1篇已发表文章 1篇已发表文章
其他PubMed (Misc)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human
已发表种属	Human
宿主/亚型	Rat / IgG1, kappa
推荐的同型对照	Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	JES3-9D7
偶联物	PE Alexa Fluor 488 Brilliant UV 737 eFluor 450 eFluor 660 Functional Grade PE-Cyanine7 PerCP-eFluor 710 Unconjugated
激发/发射光谱	查看光谱
形式	Liquid
浓度	0.2 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_466179

产品详细信息

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: JES3-9D7 has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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Oncoimmunology

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12-7108 was used in Flow cytometry/Cell sorting to investigate the impact of 5-azacytidine on xenogeneic graft-vs.-host disease and graft-vs.-leukaemia effects in a humanised murine model of transplantation.

Tue Jan 12 00:00:00 EST 2021

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

12-7108 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis.

The Journal of allergy and clinical immunology

Nguyen DX,Cotton A,Attipoe L,Ciurtin C,Doré CJ,Ehrenstein MR

12-7108 was used in Flow cytometry/Cell sorting to determine whether adalimumab's ability to boost Treg cells in vitro using PBMCs before treatment would predict clinical response.

Sat Sep 01 00:00:00 EDT 2018

Decidual T Cells Exhibit a Highly Differentiated Phenotype and Demonstrate Potential Fetal Specificity and a Strong Transcriptional Response to IFN.

Journal of immunology (Baltimore, Md. : 1950)

Powell RM,Lissauer D,Tamblyn J,Beggs A,Cox P,Moss P,Kilby MD

12-7108 was used in Flow cytometry/Cell sorting to investigate the specificity and regulation of CD4+ and CD8+ T cells obtained from human third trimester decidua and demonstrated that decidual CD4+ and CD8+ T cells exhibit a highly differentiated effector memory phenotype in comparison with peripheral blood.

Wed Nov 15 00:00:00 EST 2017

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

12-7108 was used in Microarray to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 EDT 2017

Renal Transplant Recipients Treated with Calcineurin-Inhibitors Lack Circulating Immature Transitional CD19+CD24hiCD38hi Regulatory B-Lymphocytes.

PloS one

Tebbe B,Wilde B,Ye Z,Wang J,Wang X,Jian F,Dolff S,Schedlowski M,Hoyer PF,Kribben A,Witzke O,Hoerning A

12-7108 was used in Flow cytometry/Cell sorting to analyse the effect of calcineurin inhibition on peripherally circulating regulatory B cells in renal transplant recipients receiving cyclosporine A or tacrolimus.

Mon Aug 29 00:00:00 EDT 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

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Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

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The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors.

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12-7108 was used in Flow cytometry/Cell sorting to understand immune response to FVIII in patients with HA who were either positive [HAα-FVIII(+)] or negative [HAα-FVIII(-)] for inhibitors.

Thu Feb 05 00:00:00 EST 2015

CD26-mediated induction of EGR2 and IL-10 as potential regulatory mechanism for CD26 costimulatory pathway.

Journal of immunology (Baltimore, Md. : 1950)

Hatano R,Ohnuma K,Otsuka H,Komiya E,Taki I,Iwata S,Dang NH,Okumura K,Morimoto C

12-7108 was used in Flow cytometry/Cell sorting to investigate negative feedback mechanisms to regulate CD26-mediated T cell activation.

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Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

12-7108 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

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The anti-idiotypic antibody 1F7 stimulates monocyte interleukin-10 production and induces endotoxin tolerance.

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12-7108 was used in Flow cytometry/Cell sorting to investigate the direct effects of the 1F7 mAb on peripheral blood mononuclear cells.

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Human immunology

Marino JH,Tan C,Taylor AA,Bentley C,Van De Wiele CJ,Ranne R,Paliotta M,Broughan TA,Teague TK

12-7108 was used in Flow cytometry/Cell sorting to assess IL-7 receptor expression and IL-7 signalling in human thymocytes.

Thu Apr 01 00:00:00 EDT 2010

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

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Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

生物信息学

蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

基因别名: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

UniProt ID: (Human) P22301

Entrez Gene ID: (Human) 3586

功能

参与通路

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™

产品信息

12-7108-82

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

功能检测 (FN)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

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