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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
1:50-1:100 | - |
流式细胞分析 (Flow) |
1:10 | - |
酶联免疫吸附实验 (ELISA) |
8 µg/mL | - |
功能检测 (FN) |
1:100 | - |
| 产品规格 | |
|---|---|
种属反应 |
Bovine, Human, Sheep |
宿主/亚型 |
Mouse / IgG2a |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
CC301 |
抗原 |
Recombinant bovine IL-12 |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Protein A |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2538116 |
This antibody does not react with mouse.
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer.
Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: 20C2; CLMF p35; CLMF p40; cytokine; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; Il-12; cytokine; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL35 subunit; ILN; Interleukin; interleukin 12 35kDa subunit; interleukin 12 40kDa subunit; interleukin 12 polypeptide 35; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; interleukin-12 p40 subunit; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2
基因别名: CLMF; CLMF2; IL-12; Il-12 p35; IL-12A; IL-12B; IL12; IL12A; IL12B; IL12P35; IL12p40; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35
UniProt ID: (Human) P29459, (Sheep) Q9TU27, (Bovine) P54349, (Human) P29460, (Sheep) P68220, (Bovine) P46282
Entrez Gene ID: (Human) 3592, (Sheep) 443064, (Bovine) 281856, (Human) 3593, (Sheep) 443472, (Bovine) 281857
分子生物学功能:
transmembrane signal receptor
