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IL-4 Antibody (M450B) in WB
Western blot analysis of IL-4 was performed by loading 1 µg of human recombiannt protein IL-4 (1 µg/lane, Product # RIL4I) in non-reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX). Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IL-4 was detected using a biotin conjugated IL-4 rat monoclonal antibody (Pro... View More
产品信息
M450B
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
25D2
抗原
Recombinant human IL-4 (CHO cell-derived)
偶联物
Biotin
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein G
保存液
PBS with 4% BSA
内含物
proprietary Preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_223569
产品详细信息
M450B targets IL-4 in WB and ELISA applications and shows reactivity with Human samples.
The M450B immunogen is recombinant human IL-4 (CHO cell-derived).
M450B detects IL-4 which has a predicted molecular weight of approximately 15 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
靶标信息
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
基因别名: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
microglial cell activation
innate immune response in mucosa
positive regulation of defense response to virus by host
T-helper 1 cell lineage commitment
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
chemotaxis
immune response
cellular defense response
female pregnancy
response to nutrient
cholesterol metabolic process
regulation of proton transport
negative regulation of epithelial cell migration
response to organic cyclic compound
B cell differentiation
positive regulation of B cell proliferation
B cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
response to cytokine
T-helper 2 cell cytokine production
type 2 immune response
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
regulation of phosphorylation
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
defense response to protozoan
myeloid dendritic cell differentiation
negative regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of mast cell degranulation
negative regulation of nitric oxide biosynthetic process
T-helper 2 cell differentiation
positive regulation of chemokine biosynthetic process
connective tissue growth factor biosynthetic process
regulation of isotype switching
positive regulation of MHC class II biosynthetic process
response to ethanol
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of immune response
positive regulation of sequence-specific DNA binding transcription factor activity
retina development in camera-type eye
negative regulation of white fat cell proliferation
cellular response to mercury ion
positive regulation of mononuclear cell migration
dendritic cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of myoblast fusion
negative regulation of complement-dependent cytotoxicity
negative regulation of T-helper 17 cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of eosinophil chemotaxis
negative regulation of extrinsic apoptotic signaling pathway
