Invitrogen

IQGAP2 Polyclonal Antibody

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IQGAP2 Antibody in Western Blot (WB) — Western blot analysis of IQGAP2 in Lane 1: human HeLa cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 cell lysates, Lane 4: mouse stomach tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with IQGAP2 polyclonal antibody (Product # PA5-95484) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

产品信息

PA5-95484

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	-
流式细胞分析 (Flow)	1-3 µg/1x10^6 cells	-
酶联免疫吸附实验 (ELISA)	0.1-0.5 µg/mL	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	E.coli-derived human IQGAP2 recombinant protein (Position: A1329-Q1544).
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Affinity chromatography
保存液	PBS with 4mg trehalose
内含物	0.05mg sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807286

产品详细信息

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Ras GTPase-activating-like protein IQGAP2

基因别名: 4933417J23Rik; A630053O10; AI788777; IQGAP2

UniProt ID: (Human) Q13576, (Mouse) Q3UQ44

Entrez Gene ID: (Human) 10788, (Mouse) 544963

分子生物学功能: G-protein modulator protein-binding activity modulator GTPase-activating protein

signal transduction negative regulation of GTPase activity Arp2/3 complex-mediated actin nucleation thrombin receptor signaling pathway small GTPase mediated signal transduction regulation of GTPase activity

参与通路

actin binding GTPase inhibitor activity calmodulin binding phosphatidylinositol-3,4,5-trisphosphate binding Rho GTPase binding Rac GTPase binding actin filament binding Arp2/3 complex binding GTPase activator activity