Invitrogen

IQGAP2 Polyclonal Antibody

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IQGAP2 Antibody (PA5-95484) in WB

Western blot analysis of IQGAP2 in Lane 1: human HeLa cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 cell lysates, Lane 4: mouse stomach tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with IQGAP2 polyclonal antibody (Product # PA5-95484) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP a... View More

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IQGAP2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of IQGAP2 using anti-IQGAP2 antibody (Product # PA5-95484) . IQGAP2 was detected in a section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-IQGAP2 antibody (Product # PA5-95484) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IQGAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IQGAP2 in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-IQGAP2 antibody (Product # PA5-95484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IQGAP2 Antibody in Flow Cytometry (Flow) — Flow Cytometry of IQGAP2 in SiHa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with IQGAP2 Polyclonal Antibody (Product # PA5-95484) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Product Details

PA5-95484

Applications

Tested Dilution

Publications

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

分类

Polyclonal

类型

Antibody

Immunogen

E.coli-derived human IQGAP2 recombinant protein (Position: A1329-Q1544).

View immunogen

Conjugate

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807286

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Bioinformatics

Protein Aliases: Ras GTPase-activating-like protein IQGAP2

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Gene Aliases: 4933417J23Rik; A630053O10; AI788777; IQGAP2

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UniProt ID: (Human) Q13576, (Mouse) Q3UQ44

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Entrez Gene ID: (Human) 10788, (Mouse) 544963

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Molecular Function: G-protein modulator protein-binding activity modulator GTPase-activating protein

Function(s)

signal transduction negative regulation of GTPase activity Arp2/3 complex-mediated actin nucleation thrombin receptor signaling pathway small GTPase mediated signal transduction regulation of GTPase activity

Process(es)

actin binding GTPase inhibitor activity calmodulin binding phosphatidylinositol-3,4,5-trisphosphate binding Rho GTPase binding Rac GTPase binding actin filament binding Arp2/3 complex binding GTPase activator activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IQGAP2 Polyclonal Antibody

IQGAP2 Antibody (PA5-95484) in WB

Product Details

PA5-95484

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

Species Reactivity

Host/Isotype

分类

类型

Immunogen

Conjugate

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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