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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • IQGAP2 Antibodies

          Invitrogen

          IQGAP2 Polyclonal Antibody

          View all (8) IQGAP2 antibodies
          Datasheet
          Protocols
          Tech Support

          Cite IQGAP2 Polyclonal Antibody

          Datasheet
          Protocols
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          • Antibody Testing Data (6)
          IQGAP2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          IQGAP2 Antibody (PA5-95484) in WB

          Western blot analysis of IQGAP2 in Lane 1: human HeLa cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 cell lysates, Lane 4: mouse stomach tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with IQGAP2 polyclonal antibody (Product # PA5-95484) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          PA5-95484

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          0.1-0.5 µg/mL
          -

          免疫组化(石蜡) (IHC (P))

          0.5-1 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          2 µg/mL
          -

          流式细胞分析 (Flow)

          1-3 µg/1x10^6 cells
          -

          酶联免疫吸附实验 (ELISA)

          0.1-0.5 µg/mL
          -
          产品规格

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          Immunogen

          E.coli-derived human IQGAP2 recombinant protein (Position: A1329-Q1544).
          View immunogen

          Conjugate

          Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS with 4mg trehalose

          内含物

          0.05mg sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2807286

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Target Information

          Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Ras GTPase-activating-like protein IQGAP2

          View more View less

          Gene Aliases: 4933417J23Rik; A630053O10; AI788777; IQGAP2

          View more View less

          UniProt ID: (Human) Q13576, (Mouse) Q3UQ44

          View more View less

          Entrez Gene ID: (Human) 10788, (Mouse) 544963

          View more View less

          Molecular Function: G-protein modulator protein-binding activity modulator GTPase-activating protein

          Function(s)
          signal transduction negative regulation of GTPase activity Arp2/3 complex-mediated actin nucleation thrombin receptor signaling pathway small GTPase mediated signal transduction regulation of GTPase activity
          Process(es)
          actin binding GTPase inhibitor activity calmodulin binding phosphatidylinositol-3,4,5-trisphosphate binding Rho GTPase binding Rac GTPase binding actin filament binding Arp2/3 complex binding GTPase activator activity
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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