Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
1:1,000 | - |
免疫组化(石蜡) (IHC (P)) |
1:10-1:50 | - |
染色质免疫沉淀 实验 (ChIP) |
1 µL | - |
产品规格 | |
---|---|
种属反应 |
Human, Mouse |
宿主/亚型 |
Rabbit / IgG |
分类 |
Recombinant Monoclonal |
类型 |
Antibody |
克隆号 |
19H32L12 |
抗原 |
A peptide corresponding to amino acids 579–591 of human IRAK1 |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Protein A |
保存液 |
PBS |
内含物 |
0.09% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2532270 |
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Interleukin-1 receptor-associated kinase 1 (IRAK1) is a serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. IRAK is associated with Toll-like receptor (TLR) and IL-1R signaling pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase
基因别名: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk
UniProt ID: (Human) P51617, (Mouse) Q62406
Entrez Gene ID: (Human) 3654, (Mouse) 16179