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FIGURE: 1 / 1
IRAK2 Antibody (PA5-96204) in WB
Western blot analysis of IRAK2 in extracts of Mouse liver. Samples were incubated with IRAK2 Polyclonal antibody (Product # PA5-96204) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-96204
产品规格
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IRAK2.
Conjugate
Unconjugated
形式
Liquid
浓度
0.15 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2808006
Product Specific Information
Immunogen sequence: MACYIYQLPS WVLDDLCRNM DALSEWDWME FASYVITDLT QLRKIKSMER VQGVSITREL LWWWGMRQAT VQQLVDLLCR LELYRAAQII LNWKPAPEIR CPIPAFPDSV KPEKPLAASV RKAEDEQEEG QPVRMATFPG PGSSPARAHQ PAFLQPPEED APHSLRSDLP TSSDSKDFST SIPKQEKLLS LAGDSLFWSE ADVVQATDDF NQNRKISQGT FADVYRGHRH GKPFVFKKLR ETACSSPGSI ERFFQAELQI CLRCCHPNVL; Positive Samples: Mouse liver
Target Information
IRAK2 (interleukin-1 receptor-associated kinase-like 2) binds to the IL-1 type I receptor following IL-1 engagment and triggers intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappa-B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappa-B activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappa-B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: interleukin-1 receptor-associated kinase 2c; Interleukin-1 receptor-associated kinase-like 2; IRAK-2; MGC150550; mu-IRAK-2
Gene Aliases: 6330415L08Rik; AI649099; IRAK-2; Irak2
UniProt ID: (Mouse) Q8CFA1, (Rat) Q4QQS0
Entrez Gene ID: (Mouse) 108960, (Rat) 362418
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
protein phosphorylation
signal transduction
activation of NF-kappaB-inducing kinase activity
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
intracellular signal transduction
regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
protein autophosphorylation
positive regulation of NF-kappaB transcription factor activity
interleukin-1-mediated signaling pathway
response to interleukin-1
biological_process
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