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IRF4 Antibody (711295) in ICC/IF
For immunofluorescence analysis, Ramos cells were fixed and permeabilized for detection of endogenous IRF4 using Anti- IRF4 Recombinant Rabbit Polyclonal Antibody (Product # 711295, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of IRF4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More
产品信息
711295
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
16 HCLC
抗原
Peptides corresponding to Human IRF4 (aa 425-442, 175-192)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633153
产品详细信息
This antibody is predicted to react with Monkey, Cat and Bat
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5
基因别名: IRF4; LSIRF; MUM1; NF-EM5; SHEP8
UniProt ID: (Human) Q15306
Entrez Gene ID: (Human) 3662
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein-lysine N-methyltransferase activity
sequence-specific DNA binding
winged helix/forkhead transcription factor
transcription from RNA polymerase II promoter
peptidyl-lysine methylation
negative regulation of toll-like receptor signaling pathway
T cell activation
defense response to protozoan
myeloid dendritic cell differentiation
positive regulation of DNA binding
histone H3 acetylation
histone H4 acetylation
positive regulation of interleukin-10 biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-13 biosynthetic process
positive regulation of interleukin-4 biosynthetic process
regulation of T-helper cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
T-helper 17 cell lineage commitment
