Invitrogen

ISL1 Monoclonal Antibody (1H9)

3个已发表图片

4 篇参考文献

查看其他21个ISL1抗体

问题与答案

问题与答案

抗体检测数据 (3)
已发表图片 (3)

ISL1 Antibody in Immunocytochemistry (ICC/IF)

ISL1 Antibody in Immunocytochemistry (ICC/IF)

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ISL1 Antibody (MA5-15515) in ICC/IF

Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-human IgG Fc using ISL1 monoclonal antibody (Product # MA5-15515) (Green). Blue: DRAQ5 fluorescent DNA dye.

ISL1 Antibody in Immunocytochemistry (ICC/IF)

ISL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISL1 Antibody in Western Blot (WB)

ISL1 Antibody in Immunohistochemistry (IHC)

ISL1 Antibody in Immunohistochemistry (IHC)

ISL1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ISL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-human IgG Fc using ISL1 monoclonal antibody (Product # MA5-15515) (Green). Blue: DRAQ5 fluorescent DNA dye.

ISL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right) using ISL1 monoclonal antibody (Product # MA5-15515) followed with DAB staining.

ISL1 Antibody in Western Blot (WB) — Western blot analysis of ISL1 using ISL1 monoclonal antibody (Product # MA5-15515) in full-length ISL1 (aa1-349) human IgG Fc transfected HEK293 cell lysate (1).

ISL1 Antibody in Immunohistochemistry (IHC) — Generation and identification of engineered hiPSC-derived cortical organoids. a Schematic diagram and bright-field images of hiPSC-derived cortical organoids developed on a chip. Scale bar = 200 mum. b Immunohistochemical image of tissue sections used to detect the apoptosis protein Caspase3, neural progenitor cells (NESTIN and SOX2), differentiated neurons (TUJ1), distinct brain regions (forebrain: FOXG1, PAX6; hindbrain ISL1; developing brain marker PAX2; cortical layer, TBR1 and CTIP2), inhibitory neurons (GABA) and excitatory neurons (vGLUT1) in cortical organoids at 30 and 40 days of differentiation by immunohistochemical analysis. Scale bars = 100 mum.

ISL1 Antibody in Immunohistochemistry (IHC) — Identification of specific brain regions in brain organoids. (A) Forebrain markers (PAX6, FOXG1) and hindbrain markers (PAX2, ISL1) were quantified by RT-PCR in spheroids at 10 and 16 days of differentiation. The expression values were normalized to the beta-actin expression level. Three independent experiments had been performed. Data are shown as mean +- SD. * p < 0.05, ** p < 0.01. (B) Immunohistochemical staining of PAX6 and PAX2 markers in cryosections of spheroids at 16 days and 33 days. (C) Immunohistochemical staining of PAX6 and ISL1 markers in cryosections of brain organoids at 33 days. Scale bars = 50 mum.

ISL1 Antibody in Immunohistochemistry (IHC) — Fig 5 Dysregulated the cortical layer and hindbrain populations in HSV-1-infected neuroepithelial buds. (A) The dorsal NSC marker (PAX6) and newborn neuron marker (TUJ) were observed in the brain cerebral organoids at the 18 day after 3 days with HSV-1 infection (Mock, Low infection). Scale bars: 25 mum. The images were taken Images were captured on a Leica TCS SP8 STED confocal microscope. (B) Comparison of depths of CP layer between mock- and HSV-1-infected neuroepithelial buds. (C) Immunofluorescence staining for the hindbrain marker (ISL1) in neuroepithelial buds at the day 18 after 3 days with HSV-1 infection. Scale bars: 25 mum. (D) Quantification of ISL1-positive cells in the presence or absence of HSV-1 infection. Data represent the mean +- SEM from four experiments. **p < 0.01 by Student's t test.

产品信息

MA5-15515

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化 (IHC)

-

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

-

免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human

已发表种属

Chimpanzee, Human, Non-human primate

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1H9

抗原

Purified recombinant fragment of human ISL1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. not determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

MA5-15515 targets ISL1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15515 immunogen is purified recombinant fragment of human ISL1 expressed in E. Coli.

MA5-15515 detects ISL1 which has a predicted molecular weight of approximately 39kDa.

靶标信息

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Insulin gene enhancer protein ISL-1; ISL1 transcription factor, LIM/homeodomain; Islet-1

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基因别名: Isl-1; ISL1; ISLET1

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UniProt ID: (Human) P61371

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Entrez Gene ID: (Human) 3670

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding chromatin binding protein binding zinc ion binding ligand-dependent nuclear receptor binding estrogen receptor binding bHLH transcription factor binding

negative regulation of transcription from RNA polymerase II promoter neural crest cell migration secondary heart field specification outflow tract septum morphogenesis outflow tract morphogenesis endocardial cushion morphogenesis cardiac right ventricle morphogenesis regulation of secondary heart field cardioblast proliferation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of vascular endothelial growth factor production spinal cord motor neuron cell fate specification spinal cord motor neuron differentiation visceral motor neuron differentiation trigeminal nerve development pituitary gland development pancreas development axon regeneration retinal ganglion cell axon guidance positive regulation of insulin secretion positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-1 alpha production positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production negative regulation of intracellular estrogen receptor signaling pathway positive regulation of histone acetylation positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of DNA binding negative regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter neuron fate specification mesenchymal cell differentiation sensory system development peripheral nervous system neuron axonogenesis negative regulation of inflammatory response ventricular cardiac muscle tissue morphogenesis pharyngeal system development cardiac muscle cell myoblast differentiation innervation atrial septum morphogenesis cardiac cell fate determination cellular response to glucocorticoid stimulus positive regulation of granulocyte colony-stimulating factor production negative regulation of protein homodimerization activity negative regulation of canonical Wnt signaling pathway positive regulation of macrophage colony-stimulating factor production