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Invitrogen
IkB zeta Monoclonal Antibody (hft2nap), PE, eBioscience™
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IkB zeta Antibody (12-9853-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated with recombinant human IL-2 (Product # 14-8029-81), IL-12 (Product # 14-8129-62), and IL-18 (right) for 6 hours. Cultures were then harvested, surface-stained with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42), and fixed and permeabilized with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). After fixation and permeabilization, cells were stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human I kappa B Zeta PE... View More
产品信息
12-9853-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
hft2nap
抗原
Human IkBz N-terminal Fragment (1-200)
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The hft2nap monoclonal antibody reacts with human IkB zeta (INAP, MAIL, IkBz). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with ROR alpha and ROR gamma t to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.
Applications Reported: This hft2nap antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This hft2nap antibody has been pre-titrated and tested on stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ30225; FLJ34463; I-kappa-B-zeta; Ikappa B-zeta variant 3; IkappaB-zeta; ikappaBzeta; IkB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; MAIL; Molecule possessing ankyrin repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta; OTTHUMP00000214340
基因别名: IKBZ; INAP; MAIL; NFKBIZ
UniProt ID: (Human) Q9BYH8
Entrez Gene ID: (Human) 64332
