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Invitrogen
Influenza A NP Monoclonal Antibody (D67J), FITC
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Influenza A NP Antibody (MA1-7322) in Flow
293T cells were infected with influenza PR8 virus (moi 0.001) for 24hours. Cells were harvested for FACS analysis. Anti-Influenza A NP Monoclonal Antibody, FITC Conjugate (Product # MA1-7322) was used at dilutions of 1:10, 1:20 and 1:40 to stain for influenza NP in these samples. Data courtesy of the Innovators Program.
产品信息
MA1-7322
应用
建议稀释比
已发表文章
产品规格
种属反应
Virus
已发表种属
C. elegans,
Virus
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
D67J
抗原
Influenza A Nucleoprotein.
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2, with 10mg/mL BSA
内含物
0.1% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
The MA1-7322 antibody reacts with Influenza A virus NP in viral and infected tissue samples. Reactive with all A strains.
MA1-7322 has been successfully used in immunofluorescence, FACS, and ELISA applications. The recommended starting dilution is 1:5 - 1:20 but optimal dilution should be determined by the end user. Acetone fixation of the antigen source is recommended prior to staining.
The MA1-7322 antibody was raised against Influenza A Nucleoprotein.
靶标信息
The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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