Invitrogen

Insulin Monoclonal Antibody (ICBTACLS), eBioscience™

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Insulin Antibody (14-9769-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human pancreas using 0.25 µg/mL of Mouse IgG2a K Isotype Control Purified (left) or 0.25 µg/mL of Anti-Insulin Purified (right) followed by 10 µg/mL of Anti-Mouse IgG Biotin, Avidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

Insulin Antibody in Immunocytochemistry (ICC/IF)

Insulin Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Insulin Antibody in Immunohistochemistry (IHC)

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产品信息

14-9769-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

0.25 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

ICBTACLS

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2573014

产品详细信息

Description: This ICBTACLS monoclonal antibody reacts with the hormone insulin, responsible for regulation of carbohydrate and fat metabolism. Blood glucose levels are maintained by insulin. Insulin secretion from pancreatic beta-cells within the islets of Langerhans results in uptake of excess glucose from the blood into organs and its storage in the form of glycogen. Patients with type 1 diabetes have an autoimmune destruction of insulin-producing beta-cells in the pancreas, resulting in insulin deficiency and elevated levels of blood glucose. Patients with type 2 diabetes have insulin resistance where insulin becomes less effective at reducing the amount of blood glucose. Insulin is also important for proper endothelial cell function in with respect to vascular elasticity in response to changing blood pressure and plays a role in cognition, learning, and memory.

The ICBTACLS antibody recognizes human, mouse, and rat insulin.

Applications Reported: This ICBTACLS antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This ICBTACLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 0.25 µg/mL. This ICBTACLS antibody has been tested by western blot of non-reduced pancreatic tissue lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Human proinsulin production in the milk of transgenic cattle.

Biotechnology journal

Monzani PS,Sangalli JR,Sampaio RV,Guemra S,Zanin R,Adona PR,Berlingieri MA,Cunha-Filho LFC,Mora-Ocampo IY,Pirovani CP,Meirelles FV,Wheeler MB,Ohashi OM

Published figure using Insulin monoclonal antibody (Product # 14-9769-82) in Western Blot

Fri Mar 01 00:00:00 UTC 2024

Urolithin C alleviates pancreatic β-cell dysfunction in type 1 diabetes by activating Nrf2 signaling.

Nutrition & diabetes

Luo C,Hou C,Yang D,Tan T,Chao C

14-9769-82 was used in Immunohistochemistry to explore the mechanism underlying urolithin C (UC)-mediated regulation of β-cell function.

Fri Dec 01 00:00:00 UTC 2023

Urolithin C alleviates pancreatic β-cell dysfunction in type 1 diabetes by activating Nrf2 signaling.

Nutrition & diabetes

Luo C,Hou C,Yang D,Tan T,Chao C

14-9769-82 was used in Immunohistochemistry to explore the mechanism underlying urolithin C (UC)-mediated regulation of β-cell function.

Fri Dec 01 00:00:00 UTC 2023

Alterations in hippocampal neurogenesis and hippocampal insulin signaling pathway in rat with metabolic syndrome.

Iranian journal of basic medical sciences

Bayram P,Billur D,Kizil S,Caliskan H,Can B

14-9769-82 was used in Immunohistochemistry (Paraffin), Western Blot to conclude that that the insulin signaling pathway may have an effect on the decreased neurogenesis in the MetS group.

Tue Nov 01 00:00:00 UTC 2022

Loss of urotensin II receptor diminishes hyperglycemia and kidney injury in streptozotocin-treated mice.

Journal of molecular endocrinology

Peixoto-Neves D,Kanthakumar P,Kumar R,Soni H,Adebiyi A

14-9769-82 was used in Immunohistochemistry to evaluate the effect of UT deletion on the development of hyperglycemia and diabetic kidney disease (DKD) in streptozotocin (STZ)-treated mice.

Fri Apr 01 00:00:00 UTC 2022

Exenatide prevents statin-related LDL receptor increase and improves insulin secretion in pancreatic beta cells (1.1E7) in a protein kinase A-dependent manner.

Journal of applied biomedicine

Buldak L,Machnik G,Skudrzyk E,Boldys A,Maliglowka M,Kosowski M,Basiak M,Buldak RJ,Okopien B

14-9769-82 was used in Immunocytochemistry to explore the impact of atorvastatin on pancreatic islet beta cells line (1.1.E7).

Thu Dec 01 00:00:00 UTC 2022

Effect of hTIMP-1 overexpression in human umbilical cord mesenchymal stem cells on the repair of pancreatic islets in type-1 diabetic mice.

Cell biology international

Bao Y,Zhao Z,Gao H

Published figure using Insulin monoclonal antibody (Product # 14-9769-82) in Immunocytochemistry

Sat May 01 00:00:00 UTC 2021

Pregestational diet transition to normal-fat diet avoids the deterioration of pancreatic β-cell function in male offspring induced by maternal high-fat diet.

The Journal of nutritional biochemistry

Liu Z,Ding Z,Lynch EC,McCauley N,Zhou Y,Zhang KK,Xie L

14-9769-82 was used in Immunohistochemistry to evaluate whether the adverse effects of maternal overnutrition on offspring β-cell function are reversible or preventable through preconception maternal diet interventions.