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JAM-A (CD321) Antibody (710228) in ICC/IF
Immunofluorescence analysis of JAM1/CD321 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JAM1 (24HCLC) Recombinant Rabbit Polyclonal Antibody (Product # 710228) at 2 µg/mL in 0.1% BSA and incubated for 3 hou... View More
产品信息
710228
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
24HCLC
抗原
Peptide corresponding to amino acids 290-299 of human junctional adhesion molecule-A
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532637
产品详细信息
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD321; F11 R; JAM-1; JAM-A; Junctional adhesion molecule 1; Junctional adhesion molecule A; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor
基因别名: CD321; F11R; JAM; JAM1; JAMA; JCAM; KAT; PAM-1; UNQ264/PRO301
UniProt ID: (Human) Q9Y624
Entrez Gene ID: (Human) 50848
regulation of cytokine production
inflammatory response
transforming growth factor beta receptor signaling pathway
response to radiation
extracellular matrix organization
actomyosin structure organization
negative regulation of GTPase activity
positive regulation of GTPase activity
positive regulation of blood pressure
viral entry into host cell
intestinal absorption
leukocyte migration
bicellular tight junction assembly
establishment of protein localization to plasma membrane
establishment of endothelial intestinal barrier
regulation of membrane permeability
cell-cell adhesion
regulation of actin cytoskeleton reorganization
