Invitrogen

JMJD2C Polyclonal Antibody

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JMJD2C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of JMJD2C using a polyclonal antibody (Product # PA5-23065).

JMJD2C Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of JMJD2C using a polyclonal antibody (Product # PA5-23065).

JMJD2C Antibody in Western Blot (WB) — Western blot analysis of JMJD2C using a polyclonal antibody (Product # PA5-23065).

JMJD2C Antibody — Figure 3. PITX1 regulates JMJD2B and JMJD2C expression in a HIF-1alpha-dependent manner. (A) HeLa cells were transfected with control or PITX1 siRNA, prior to treatment with 1% O2 for 24 hours. Total mRNA was extracted and levels of JMJD2A, JMJD2B and JMJD2C mRNA were analyzed by qPCR. Graph depicts mean and standard deviation of a minimum of 3 independent experiments. Data was normalized using actin and compared to control siRNA. Student's t-test was performed to calculate p values, and levels of significance are denoted as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001. (B) HeLa cells were transfected with control or HIF-1alpha siRNA, prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by protein gel blot using the indicated antibodies. (C) HeLa cells were transfected with control, PITX1, HIF-1a or PITX1 and HIF-1alpha siRNA, prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by western blot using the indicated antibodies. (D) HeLa cells were transfected with the indicated siRNAs oligonucleotides prior to treatment with 1% O2 for 24 hours. Whole cell lysates were obtained 48 hours post-transfection and analyzed by protein gel blot using the indicated antibodies. {TM}

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:10,000

View 2 publications 2 publications

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100

染色质免疫沉淀实验 (ChIP)

Assay-Dependent

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide made to an internal region of the human JMJD2C protein (within residues 450-600).

偶联物

Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152049

产品详细信息

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

靶标信息

Jumonji domain-containing protein 2C (JMJD2C)/ Lysine-specific demethylase 4C (KDM4C) is a histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3; thereby, playing a central role in histone code. KDM4C does not demethylate histone H3 'Lys-4', H3 'Lys-27', or H4 'Lys-20'. It demethylates trimethylated H3 "Lys-9' and H3 "Lys-36' residues. There is no activity on mono- or dimethylated residues. Demethylation of Lys residues generates formaldehyde and succinate.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: [histone H3]-trimethyl-L-lysine(9) demethylase 4C; bA146B14.1; GASC-1 protein; gene amplified in squamous cell carcinoma 1; Gene amplified in squamous cell carcinoma 1 protein; JmjC domain-containing histone demethylation protein 3C; jumonji domain containing 2C; Jumonji domain-containing protein 2C; lysine (K)-specific demethylase 4C; Lysine-specific demethylase 4C; RP11-169L18.1; tudor domain containing 14C

基因别名: 2410141F18Rik; AA517467; GASC1; JHDM3C; JMJD2C; KDM4C; KIAA0780; TDRD14C

UniProt ID: (Human) Q9H3R0, (Mouse) Q8VCD7

Entrez Gene ID: (Human) 23081, (Mouse) 76804

分子生物学功能: histone modifying enzyme