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Invitrogen
KEAP1 Polyclonal Antibody
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图: 1 / 6
KEAP1 Antibody (PA5-23601) in ICC/IF
Immunofluorescence analysis of Kelch-like ECH-associated protein 1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.01% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KEAP1 Polyclonal Antibody (Product # PA5-23601) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-23601
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 422-449 amino acids from the C-terminal region of human KEAP1
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
200 µg
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is predicted to react with porcine based on sequence homology.
靶标信息
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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