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Invitrogen
KIF15 Polyclonal Antibody
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KIF15 Antibody (PA5-143949) in ICC/IF
Immunocytochemistry analysis of KIF15 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in KIF15 Polyclonal antibody (Product # PA5-143949) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
产品信息
PA5-143949
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human KIF15 recombinant protein (Position: M1201-S1388).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human U20S whole cell, human K562 whole cell, human PC-3 whole cell, human HepG2 whole cell, human Hela whole cell. IHC: human colon cancer tissue, rat testis tissue. ICC/IF: A431 cell. Flow: SiHa cell, U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly. Target protein's mouse homolog associates with microtubulew and appears to play a role in mitosis in fibroblasts and in migration in developing neurons.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hKLP2; kinesin-like 7; Kinesin-like protein 2; Kinesin-like protein 7; Kinesin-like protein KIF15; Serologically defined breast cancer antigen NY-BR-62
基因别名: HKLP2; KIF15; KLP2; KNSL7; NY-BR-62
UniProt ID: (Human) Q9NS87, (Rat) Q7TSP2
Entrez Gene ID: (Human) 56992, (Rat) 353302
